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Vesicle Fusion Studied by Surface Plasmon Resonance and Surface Plasmon Fluorescence Spectroscopy

机译:表面等离子体共振和表面等离子体荧光光谱研究囊泡融合

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摘要

Substrate-supported planar lipid bilayers are generated most commonly by the adsorption and transformation of phospholipid vesicles (vesicle fusion). We have recently demonstrated that simultaneous measurements of surface plasmon resonance (SPR) and surface plasmon fluorescence spectroscopy (SPFS) are highly informative for monitoring lipid membranes on solid substrates. SPR and SPFS provide information on the amount and topography of adsorbed lipid membranes, respectively. In this study, the vesicle fusion process was studied in detail by measuring SPR-SPFS at a higher rate and plotting the obtained fluorescence intensity versus film thickness. We could track the initial adsorption of vesicles, the onset of vesicle rupture occurring at certain vesicle coverage of the surface, and the autocatalytic transformation into planar bilayers. We also monitored vesicle fusion of the same vesicle suspensions by quartz crystal microbalance with dissipation monitoring (QCM-D). We compared the results obtained from SPR-SPFS and QCM-D to highlight the unique information provided by SPR-SPFS.
机译:基质支持的平面脂质双层最常见地通过磷脂囊泡的吸附和转化(囊泡融合)产生。我们最近证明,同时测量表面等离子体共振(SPR)和表面等离子体荧光光谱(SPFS)对于监测固体基质上的脂质膜非常有用。 SPR和SPFS分别提供了有关脂质膜吸附量和形貌的信息。在这项研究中,通过以较高的速率测量SPR-SPFS并绘制获得的荧光强度与膜厚度的关系图,对囊泡融合过程进行了详细的研究。我们可以跟踪囊泡的初始吸附,在一定的囊泡表面覆盖范围内发生的囊泡破裂的发生以及自催化转变为平面双层的过程。我们还通过耗散监测(QCM-D)通过石英晶体微量天平监测了相同囊泡悬浮液的囊泡融合。我们比较了从SPR-SPFS和QCM-D获得的结果,以突出显示SPR-SPFS提供的独特信息。

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