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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Integrated Metabolome and Transcriptome Analysis Provide Insights into the Effects of Grafting on Fruit Flavor of Cucumber with Different Rootstocks
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Integrated Metabolome and Transcriptome Analysis Provide Insights into the Effects of Grafting on Fruit Flavor of Cucumber with Different Rootstocks

机译：整合的代谢组和转录组分析提供了不同嫁接砧木嫁接对黄瓜果实风味的影响的见解

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Rootstocks frequently exert detrimental effects on the fruit quality of grafted cucumber (Cucumis sativus L.) plants. To understand and ultimately correct this deficiency, a transcriptomic and metabolomic comparative analysis was performed among cucumber fruits from non-grafted plants (NG), and fruits from plants grafted onto different rootstocks of No.96 and No.45 (Cucurbita moschata. Duch), known to confer a different aroma and taste. We found remarkable changes in the primary metabolites of sugars, organic acids, amino acids, and alcohols in the fruit of the grafted cucumber plants with different rootstocks, compared to the non-grafted ones, especially No.45. We identified 140, 131, and 244 differentially expressed genes (DEGs) in the comparisons of GNo.96 vs. NG, GNo.45 vs. NG, and GNo.45 vs. GNo.96. The identified DEGs have functions involved in many metabolic processes, such as starch and sucrose metabolism; the biosynthesis of diterpenoid, carotenoid, and zeatin compounds; and plant hormone signal transduction. Members of the HSF, AP2/ERF-ERF, HB-HD-ZIP, and MYB transcription factor families were triggered in the grafted cucumbers, especially in the cucumber grafted on No.96. Based on a correlation analysis of the relationships between the metabolites and genes, we screened 10 candidate genes likely to be involved in sugar metabolism (Fructose-6-phosphate and trehalose), linoleic acid, and amino-acid (isoleucine, proline, and valine) biosynthesis in grafted cucumbers, and then confirmed the gene expression patterns of these genes by qRT-PCR. The levels of TPS15 (Csa3G040850) were remarkably increased in cucumber fruit with No.96 rootstock compared with No.45, suggesting changes in the volatile chemical production. Together, the results of this study improve our understanding of flavor changes in grafted cucumbers, and identify the candidate genes involved in this process.
机译：砧木经常对嫁接黄瓜（Cucumis sativus L.）植物的果实品质产生有害影响。为了理解并最终纠正这种缺陷，在非嫁接植物（NG）黄瓜果实和嫁接到96号和45号不同砧木（南瓜属）上的植物果实之间进行了转录组和代谢组学比较分析。 ，已知会带来不同的香气和味道。我们发现，与未嫁接的砧木相比，嫁接的黄瓜具有不同砧木的果实中糖，有机酸，氨基酸和醇的主要代谢产物发生了显着变化，尤其是No.45。在GNo.96与NG，GNo.45与NG以及GNo.45与GNo.96的比较中，我们鉴定了140、131和244个差异表达基因（DEG）。所确定的DEG具有涉及许多代谢过程的功能，例如淀粉和蔗糖代谢；二萜，类胡萝卜素和玉米素化合物的生物合成；和植物激素信号转导。在嫁接的黄瓜中，特别是在96号嫁接的黄瓜中，触发了HSF，AP2 / ERF-ERF，HB-HD-ZIP和MYB转录因子家族的成员。基于对代谢物和基因之间关系的相关分析，我们筛选了可能与糖代谢（果糖6-磷酸和海藻糖），亚油酸和氨基酸（异亮氨酸，脯氨酸和缬氨酸）有关的10个候选基因。 ）在嫁接黄瓜中进行生物合成，然后通过qRT-PCR确认这些基因的基因表达模式。 96号砧木的黄瓜果实中的TPS15（Csa3G040850）水平显着高于45号黄瓜，表明挥发性化学物质生产发生了变化。总之，这项研究的结果增进了我们对嫁接黄瓜风味变化的理解，并确定了参与此过程的候选基因。

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