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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Comparative Transcriptome Analysis of Developing Seeds and Silique Wall Reveals Dynamic Transcription Networks for Effective Oil Production in Brassica napus L.
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Comparative Transcriptome Analysis of Developing Seeds and Silique Wall Reveals Dynamic Transcription Networks for Effective Oil Production in Brassica napus L.

机译：发育种子和长角果壁的比较转录组分析揭示了有效转录的甘蓝型油菜动态转录网络。

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Vegetable oil is an essential constituent of the human diet and renewable raw material for industrial applications. Enhancing oil production by increasing seed oil content in oil crops is the most viable, environmentally friendly, and sustainable approach to meet the continuous demand for the supply of vegetable oil globally. An in-depth understanding of the gene networks involved in oil biosynthesis during seed development is a prerequisite for breeding high-oil-content varieties. Rapeseed (Brassica napus) is one of the most important oil crops cultivated on multiple continents, contributing more than 15% of the world’s edible oil supply. To understand the phasic nature of oil biosynthesis and the dynamic regulation of key pathways for effective oil accumulation in B. napus, comparative transcriptomic profiling was performed with developing seeds and silique wall (SW) tissues of two contrasting inbred lines with ~13% difference in seed oil content. Differentially expressed genes (DEGs) between high- and low-oil content lines were identified across six key developmental stages, and gene enrichment analysis revealed that genes related to photosynthesis, metabolism, carbohydrates, lipids, phytohormones, transporters, and triacylglycerol and fatty acid synthesis tended to be upregulated in the high-oil-content line. Differentially regulated DEG patterns were revealed for the control of metabolite and photosynthate production in SW and oil biosynthesis and accumulation in seeds. Quantitative assays of carbohydrates and hormones during seed development together with gene expression profiling of relevant pathways revealed their fundamental effects on effective oil accumulation. Our results thus provide insights into the molecular basis of high seed oil content (SOC) and a new direction for developing high-SOC rapeseed and other oil crops.
机译：植物油是人类饮食中不可或缺的成分，是工业应用中的可再生原料。通过增加油料作物中的籽油含量来提高石油产量是满足全球对植物油供应持续需求的最可行，最环保和可持续的方法。对种子发育过程中与油生物合成有关的基因网络的深入了解是育种高油含量品种的先决条件。油菜籽（甘蓝型油菜）是在多个大洲种植的最重要的油料作物之一，占世界食用油供应量的15％以上。为了了解油生物合成的阶段性和对甘蓝型油菜有效油积累的关键途径的动态调节，对发育中的种子和长角较薄的两个自交系自交系（SW）组织进行了比较转录组谱分析，差异约为13％。种子油含量。高油含量和低油含量之间的差异表达基因（DEGs）在六个关键的发育阶段得到鉴定，基因富集分析表明，与光合作用，代谢，碳水化合物，脂质，植物激素，转运蛋白，三酰甘油和脂肪酸合成有关的基因往往在高含油量线中被上调。揭示了差异调节的DEG模式，用于控制西南部的代谢物和光合产物的生产以及种子中油的生物合成和积累。种子发育过程中糖类和激素的定量分析以及相关途径的基因表达谱分析显示了它们对有效油脂积累的基本作用。因此，我们的结果为深入了解高种子油含量（SOC）的分子基础，以及为发展高SOC油菜籽和其他油料作物的新方向提供了见识。

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