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Resistance of Bacterial Endospores to Outer Space for Planetary Protection Purposes—Experiment PROTECT of the EXPOSE-E Mission

机译:出于细菌保护目的细菌内生孢子对太空的抵抗力— EXPOSE-E任务的实验保护

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摘要

Spore-forming bacteria are of particular concern in the context of planetary protection because their tough endospores may withstand certain sterilization procedures as well as the harsh environments of outer space or planetary surfaces. To test their hardiness on a hypothetical mission to Mars, spores of Bacillus subtilis 168 and Bacillus pumilus SAFR-032 were exposed for 1.5 years to selected parameters of space in the experiment PROTECT during the EXPOSE-E mission on board the International Space Station. Mounted as dry layers on spacecraft-qualified aluminum coupons, the “trip to Mars” spores experienced space vacuum, cosmic and extraterrestrial solar radiation, and temperature fluctuations, whereas the “stay on Mars” spores were subjected to a simulated martian environment that included atmospheric pressure and composition, and UV and cosmic radiation. The survival of spores from both assays was determined after retrieval. It was clearly shown that solar extraterrestrial UV radiation (λ≥110 nm) as well as the martian UV spectrum (λ≥200 nm) was the most deleterious factor applied; in some samples only a few survivors were recovered from spores exposed in monolayers. Spores in multilayers survived better by several orders of magnitude. All other environmental parameters encountered by the “trip to Mars” or “stay on Mars” spores did little harm to the spores, which showed about 50% survival or more. The data demonstrate the high chance of survival of spores on a Mars mission, if protected against solar irradiation. These results will have implications for planetary protection considerations. Key Words: Planetary protection—Bacterial spores—Space experiment—Simulated Mars mission. Astrobiology 12, 445–456.
机译:形成孢子的细菌在行星保护的背景下特别受到关注,因为它们坚韧的内生孢子可以承受某些灭菌程序以及外太空或行星表面的恶劣环境。为了测试对火星假想任务的耐受性,在国际空间站执行EXPOSE-E任务期间,枯草芽孢杆菌168和短小芽孢杆菌SAFR-032的孢子在实验PROTECT中暴露了1.5年的选定空间参数。 “干燥的火星”孢子以干燥层的形式安装在太空飞船合格的铝质试样上,经历了太空真空,宇宙和外星太阳辐射以及温度波动,而“停留在火星”的孢子则受到模拟的火星环境的影响,其中包括大气压力和成分,以及紫外线和宇宙辐射。取回后测定两种测定的孢子存活率。清楚地表明,太阳外星紫外线(λ≥110nm)和火星紫外线光谱(λ≥200nm)是应用的最有害的因素。在某些样品中,只有暴露于单层的孢子中仅有少数幸存者。多层孢子的存活率提高了几个数量级。 “去火星”或“在火星上停留”孢子遇到的所有其他环境参数对孢子的危害很小,表明存活率约为50%或更高。数据表明,如果免受阳光照射,孢子在火星任务中存活的可能性很高。这些结果将对行星保护的考虑产生影响。关键字:行星保护-细菌孢子-太空实验-模拟火星任务。天体生物学12,445–456。

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