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首页> 美国卫生研究院文献>Thorax >Laser light scattering spectroscopy: a new method to measure tracheobronchial mucociliary activity.
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Laser light scattering spectroscopy: a new method to measure tracheobronchial mucociliary activity.

机译：激光散射光谱法：一种测量气管支气管粘膜纤毛活性的新方法。

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摘要

Laser light scattering spectroscopy is based on the evaluation of the frequency shift of coherent light scattered by moving particles. This makes it particularly suitable for use in light guiding systems. In this study laser light scattering spectroscopy was assessed for its ability to provide information on the motility of respiratory cilia. In vitro and in vivo measurements were undertaken with animal tracheal mucosa. The intensity fluctuations of laser light scattered from moving cilia were analysed in terms of their autocorrelation functions to provide information on the frequency and synchrony of beating cilia. In vitro measurements were performed on fresh bovine trachea to estimate a safe laser power level for mucosal exposure and to test the method by defining the temperature dependence of the ciliary beat frequency. Power densities not exceeding 0.3 kW/cm2 were found to be the upper limit for long term exposure of the mucosa in vitro. Ciliary beat frequency showed a pronounced temperature dependence, ranging from 5.8 to 28.3 Hz over the temperature range 20-43.5 degrees C. A maximum frequency was found at 41.5 degrees C. In vivo measurements of ciliary activity were performed in six pigs by means of optical fibres for light transmission combined with fibreoptic bronchoscopy. A ciliary beat frequency of 5 Hz was obtained; heart and breathing frequencies could be separated and identified. These results suggest that laser light scattering spectroscopy might provide a convenient method of studying the mucociliary system of the lower airways.
机译：激光散射光谱法是基于对运动颗粒散射的相干光的频移的评估。这使其特别适合在光导系统中使用。在这项研究中，评估了激光散射光谱技术提供有关呼吸道纤毛运动信息的能力。用动物气管粘膜进行体外和体内测量。从运动纤毛散射的激光强度起伏的自相关函数进行了分析，以提供有关跳动纤毛的频率和同步性的信息。在新鲜的牛气管上进行了体外测量，以评估粘膜暴露的安全激光功率水平，并通过定义睫状跳动频率的温度依赖性来测试该方法。发现功率密度不超过0.3 kW / cm2是体外长期暴露于粘膜的上限。纤毛搏动频率显示出明显的温度依赖性，在20-43.5摄氏度的温度范围内，范围为5.8至28.3 Hz。发现最大频率为41.5摄氏度。通过光学手段对六头猪进行了体内纤毛活性测量纤维结合光导纤维支气管镜检查。睫状跳动频率为5 Hz；心脏和呼吸频率可以分开和识别。这些结果表明，激光散射光谱法可能为研究下呼吸道粘膜纤毛系统提供一种方便的方法。

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