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首页> 美国卫生研究院文献>Cells Tissues Organs >Breast and Abdominal Adipose Multipotent Mesenchymal Stromal Cells and Stage Specific Embryonic Antigen 4 Expression
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Breast and Abdominal Adipose Multipotent Mesenchymal Stromal Cells and Stage Specific Embryonic Antigen 4 Expression

机译：乳房和腹部脂肪间充质基质细胞和阶段特异性胚胎抗原4表达。

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We assessed human mesenchymal stem cells (MSCs) harvested from breast and abdominal adipose tissues enriched in embryonic stage specific antigen (SSEA-4) expression for osteogenic and adipogenic differentiation in comparison to a mixed cell population. Human adipose was obtained from abdominal and breast tissues of females undergoing gastric bypass and breast reduction respectively. SSEA-4 expressing cells were enriched from the mixed cell population by magnetic cell sorting and expanded in culture. The results showed that freshly isolated cells from breast and abdominal tissues based on adipose from 3 patients comprised 12% and 10% SSEA-4+ cells respectively. At passage 0, 48 % of the cells from breast adipose tissue were positive for SSEA-4 while 12% of the cells from abdominal adipose tissue were positive for this antigen. The level of SSEA-4 expressing cells remained relatively constant with passaging; SSEA-4 expressing cells from breast tissue comprised 45 % of the total while 27% of the cells from abdominal adipose tissue expressed SSEA-4 at passage 5. Cells sorted for SSEA-4 expression exhibited a higher potential for differentiation toward osteogenic and adipogenc cell lineages in vitro when compared to a mixed population. Interestingly, SSEA-4 expression was lost upon differentiation suggesting that the antigen marks a subpopulation of MSCs. Taken together, the data demonstrate that breast adipose tissue is highly enriched in a subpopulation of MSCs expressing SSEA-4 and suggests that SSEA-4 may be a marker of a subpopulation of MSCs with high potential for osteogenic and adipogenic differentiation.
机译：我们评估了从混合有丰富的胚胎阶段特异性抗原（SSEA-4）表达的乳房和腹部脂肪组织中收获的人间充质干细胞（MSC），与混合细胞群体相比，它们具有成骨和成脂分化作用。人脂肪分别来自经历胃旁路手术和乳房复位的女性的腹部和乳房组织。通过磁性细胞分选从混合细胞群中富集表达SSEA-4的细胞，并在培养中扩增。结果显示，以3名患者的脂肪为基础，从乳房和腹部组织中分离得到的新鲜细胞分别包含12％和10％的SSEA-4 +细胞。在第0代时，来自乳房脂肪组织的细胞中48％的SSEA-4呈阳性，而来自腹部脂肪组织的细胞中12％的该抗原呈阳性。 SSEA-4表达细胞的水平随着传代而保持相对恒定；来自乳腺组织的SSEA-4表达细胞占总数的45％，而来自腹部脂肪组织的27％细胞在第5代表达SSEA-4。分类为SSEA-4表达的细胞表现出更高的分化成骨细胞和成脂细胞的潜力与混合人群相比，体外血统。有趣的是，SSEA-4表达在分化时丢失，表明该抗原标志着MSC的亚群。综上所述，数据表明，乳房脂肪组织高度富含表达SSEA-4的MSC亚群，并表明SSEA-4可能是具有高度成骨和成脂分化潜能的MSC亚群的标志物。

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