• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Cells Tissues Organs >On the Formation and Functions of High and Very High Magnesium Calcites in the Continuously Growing Teeth of the Echinoderm Lytechinus variegatus: Development of Crystallinity and Protein Involvement
【2h】

On the Formation and Functions of High and Very High Magnesium Calcites in the Continuously Growing Teeth of the Echinoderm Lytechinus variegatus: Development of Crystallinity and Protein Involvement

机译：棘皮动物棘齿rm连续生长牙齿中高方解石和高方解石的形成和功能：结晶性和蛋白质参与的发展

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sea urchin teeth grow continuously and develop a complex mineralized structure consisting of spatially separate but crystallographically aligned first stage calcitic elements of high Mg content (5–15 mol% mineral). These become cemented together by epitaxially oriented second stage very high Mg calcite (30–40 mol% mineral). In the tooth plumula, ingressing preodontoblasts create layered cellular syncytia. Mineral deposits develop within membrane-bound compartments between cellular syncytial layers. We seek to understand how this complex tooth architecture is developed, how individual crystalline calcitic elements become crystallographically aligned, and how their Mg composition is regulated. Synchrotron microbeam X-ray scattering was performed on live, freshly dissected teeth. We observed that the initial diffracting crystals lie within independent syncytial spaces in the plumula. These diffraction patterns match those of mature tooth calcite. Thus, the spatially separate crystallites grow with the same crystallographic orientation seen in the mature tooth. Mineral-related proteins from regions with differing Mg contents were isolated, sequenced, and characterized. A tooth cDNA library was constructed, and selected matrix-related proteins were cloned. Antibodies were prepared and used for immunolocaliztion. Matrix-related proteins are acidic, phosphorylated, and associated with the syncytial membranes. Time-of-flight secondary ion mass spectroscopy of various crystal elements shows unique amino acid, Mg, and Ca ion distributions. High and very high Mg calcites differ in Asp content. Matrix-related proteins are phosphorylated. Very high Mg calcite is associated with Asp-rich protein, and it is restricted to the second stage mineral. Thus, the composition at each part of the tooth is related to architecture and function.
机译：海胆牙齿不断生长并形成复杂的矿化结构，该结构由空间分离但晶体排列一致的高镁含量（5-15 mol％矿物）的第一阶段钙质元素组成。这些被外延取向的第二阶段的非常高的镁方解石（30-40 mol％的矿物）粘合在一起。在牙小管中，牙周成牙细胞进入会形成分层的细胞合胞体。矿物质沉积物在细胞合胞体层之间的膜结合隔室内形成。我们试图了解这种复杂的牙齿结构是如何发展的，单个晶体钙元素如何在晶体学上排列，以及它们的镁组成如何受到调节。同步辐射微束X射线散射是在鲜活的新鲜牙齿上进行的。我们观察到，最初的衍射晶体位于毛孔中独立的合胞空间内。这些衍射图谱与成熟的方解石相匹配。因此，空间上分离的微晶以在成熟牙齿中看到的相同的晶体学取向生长。分离，测序和表征了来自镁含量不同区域的矿物质相关蛋白。构建牙齿cDNA文库，并克隆选择的基质相关蛋白。制备抗体并将其用于免疫定位。基质相关蛋白是酸性的，磷酸化的，并与合胞膜结合。各种晶体元素的飞行时间二次离子质谱显示出独特的氨基酸，Mg和Ca离子分布。高和非常高的镁方解石的Asp含量不同。基质相关蛋白被磷酸化。镁含量高的方解石与富含Asp的蛋白质有关，并且仅限于第二阶段的矿物。因此，牙齿各部位的成分与结构和功能有关。

著录项

相似文献

  • 外文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号