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Morphological and Extracellular Matrix Changes following Vocal Fold Injury in Mice

机译：小鼠声带损伤后的形态学和细胞外基质变化

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Mouse experimental models are commonly utilized tools in biomedical research but remain underrepresented in vocal fold biology, presumably due to the small size of the larynx and limited description of the anatomical, cellular and extracellular composition of the vocal folds. In this study, we provide a whole-mount serial section-based histological description of vocal fold morphology of wild-type FVB strain mice, alongside a histological and immunohistochemical (IHC)-based quantitative analysis of extracellular matrix (ECM) alteration 1, 7, 14, 28, 42 and 56 days following unilateral vocal fold injury. IHC was specific for procollagen type I, collagen type I, collagen type III, collagen type IV, elastin, decorin, fibronectin and hyaluronic acid binding protein 2. The histological description confirmed the presence of a laryngeal alar structural complex in the mouse, which appears to be a morphological feature unique to rodents. The lamina propria appeared uniform without evidence of a distinct layer structure as has been reported in larger animals and humans. Time-dependent alterations in vocal fold morphology, ECM organization and ECM protein/glycoconjugate abundance were observed in injured vocal folds compared to control. The presence of a mature scar was observed between 28 and 42 days postinjury. Morphological and ECM changes following vocal fold injury in the mouse were generally consistent with those reported in other animal models, particularly the rat, although wound repair in the mouse appears to occur at a faster rate.Copyright © 2010 S. Karger AG, Basel
机译：小鼠实验模型是生物医学研究中常用的工具，但在声带生物学中仍未得到足够的代表，这可能是由于喉头的尺寸较小以及声带的解剖，细胞和细胞外组成的描述有限。在这项研究中，我们为野生型FVB品系小鼠的声带形态提供了基于全序列系列切片的组织学描述，以及基于组织学和免疫组织化学（IHC）的细胞外基质（ECM）改变1、7定量分析单侧声带损伤后第14、28、42和56天。 IHC特异于I型胶原蛋白，I型胶原蛋白，III型胶原蛋白，IV型胶原蛋白，弹力蛋白，decorin，纤连蛋白和透明质酸结合蛋白2。组织学描述证实小鼠中存在喉头阿拉尔结构复合物，该结构出现成为啮齿动物特有的形态特征。正如在大型动物和人类中所报道的那样，固有层显得均匀，没有明显的层结构证据。与对照相比，在受伤的声带中观察到声带形态，ECM组织和ECM蛋白/糖缀合物丰度随时间的变化。受伤后28至42天之间观察到成熟疤痕的存在。小鼠声带损伤后的形态学和ECM变化通常与其他动物模型（尤其是大鼠）中报道的一致，尽管小鼠的伤口修复似乎以更快的速度发生。版权所有©2010 S.Karger AG，巴塞尔

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