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首页> 美国卫生研究院文献>Experimental and Therapeutic Medicine >Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas
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Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas

机译：平阳霉素对海绵状血管瘤的促凋亡作用及作用机制

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摘要

This study aimed to investigate the proapoptotic effects and the mechanism of action of pingyangmycin (PY) on cavernous hemangioma. The rat spleen was used as a model of cavernous hemangioma. PY was injected into the spleen and the pathological changes were observed at different time-points. Apoptosis was detected using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and transmission electron microscopy (TEM). The expression levels of the apoptosis-related protein, caspase-3, were determined using immunohistochemistry and image analysis. Rats injected with normal saline were the control group. Injection of normal saline did not damage rat spleens. On days 2 and 5 following PY injection, the spleens exhibited slight swelling. On days 8 and 14, atrophic changes were observed and the splenic sinus endothelial cells were damaged. At various time-points following PY injection, the apoptotic cells were observed by TEM. The TUNEL assay showed that apoptosis occurred widely among the splenic sinus endothelial cells and other splenic cells. The apoptotic rate and caspase-3 expression levels increased with prolonged PY exposure. PY induced apoptosis of splenic sinus endothelial cells through the caspase-3 activation pathway, and resulted in endothelial cell necrosis and fibroblast hyperplasia.
机译：本研究旨在探讨平阳霉素（PY）对海绵状血管瘤的促凋亡作用及其作用机制。大鼠脾脏被用作海绵状血管瘤的模型。将PY注射入脾脏并在不同时间点观察到病理变化。使用末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）分析和透射电子显微镜（TEM）检测凋亡。使用免疫组织化学和图像分析确定凋亡相关蛋白caspase-3的表达水平。注射生理盐水的大鼠为对照组。注射生理盐水不会损害大鼠脾脏。 PY注射后第2天和第5天，脾脏出现轻微肿胀。在第8天和第14天，观察到萎缩性变化，脾窦内皮细胞受损。在PY注射后的各个时间点，通过TEM观察凋亡细胞。 TUNEL分析显示，脾窦内皮细胞和其他脾细胞中广泛发生凋亡。细胞凋亡率和caspase-3表达水平随着PY暴露时间的延长而增加。 PY通过caspase-3激活途径诱导脾窦内皮细胞凋亡，并导致内皮细胞坏死和成纤维细胞增生。

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