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首页> 美国卫生研究院文献>Experimental and Therapeutic Medicine >Combined analysis of serum γ-glutamyl transferase isoenzyme II α-L-fucosidase and α-fetoprotein detected using a commercial kit in the diagnosis of hepatocellular carcinoma
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Combined analysis of serum γ-glutamyl transferase isoenzyme II α-L-fucosidase and α-fetoprotein detected using a commercial kit in the diagnosis of hepatocellular carcinoma

机译：使用商业试剂盒检测的血清γ-谷氨酰转移酶同工酶IIα-L-岩藻糖苷酶和α-甲胎蛋白的组合分析在肝细胞癌的诊断中

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γ-glutamyl transferase isoenzyme II (GGT-II) is a sensitive biomarker of hepatocellular carcinoma (HCC). However, numerous disadvantages of the traditional manual method affected its application. The commercial kit provided a convenient and fast method for the determination of GGT-II levels. The purposes of the present study were to compare the reproducibility and sensitivity between the manual and commercial kit methods and to evaluate the diagnostic efficiency for HCC with the combined analysis of GGT-II, α-L-fucosidase (AFU) and α-fetoprotein (AFP). In patients with various liver diseases (HCC, liver cirrhosis and chronic hepatitis) and normal subjects, GGT-II was detected by manual and commercial polyacrylamide gel electrophoresis (PAGE). The levels of AFU and AFP were assayed by colorimetry and a chemiluminescence immunoassay, respectively. The commercial PAGE had equal diagnostic efficiency with traditional manual PAGE and no significant differences were observed in intra- and average-gel reproducibility and GGT-II sensitivities between the manual and commercial PAGE (P>0.05). The incidence of GGT-II detected by commercial PAGE in HCC patients was 84.1% and <8% in benign liver disease. The levels of AFU and AFP in the benign liver diseases and normal subjects were lower than those in HCC. According to the cut-off value obtained by receiver operating characteristic curves, a total of 56.6 and 59.3% of HCC patients (64 out of 113 and 67 out of 113) had AFU >636.5 μmol/l h and AFP >44.0 μg/l, respectively. There were no significant correlations between GGT-II and AFU or AFP. Combined detection of GGT-II with AFU or AFP increased the diagnostic sensitivity to 92.9 and 93.8%, respectively. These results suggest that commercial PAGE provides a simple and reproducible method for GGT-II detection. Combined determination of GGT-II with AFU or AFP exhibited superior sensitivity and specificity for the diagnosis of HCC.
机译：γ-谷氨酰转移酶同工酶II（GGT-II）是肝细胞癌（HCC）的敏感生物标志物。但是，传统手动方法的许多缺点影响了其应用。商业试剂盒为测定GGT-II水平提供了一种方便快捷的方法。本研究的目的是比较手动试剂盒方法和商业试剂盒方法之间的可重复性和敏感性，并通过结合分析GGT-II，α-L-岩藻糖苷酶（AFU）和α-甲胎蛋白来评估HCC的诊断效率（法新社）。在患有各种肝病（HCC，肝硬化和慢性肝炎）和正常受试者的患者中，通过手动和商用聚丙烯酰胺凝胶电泳（PAGE）检测到GGT-II。通过比色法和化学发光免疫法分别测定AFU和AFP的水平。商用PAGE与传统手动PAGE的诊断效率相同，并且在手动和商用PAGE之间在凝胶内和平均凝胶重现性以及GGT-II敏感性方面没有观察到显着差异（P> 0.05）。在良性肝病中，通过商业PAGE检测到的HCC患者中GGT-II的发生率为84.1％，<8％。肝良性疾病和正常人的AFU和AFP水平低于肝癌。根据受试者工作特征曲线获得的临界值，共有56.6％和59.3％的HCC患者（113名中的64名和113名中的67名）的AFU> 636.5μmol/ lh，AFP> 44.0μg/ l，分别。 GGT-II与AFU或AFP之间无显着相关性。与AFU或AFP一起检测GGT-II的诊断敏感性分别提高到92.9和93.8％。这些结果表明，商业PAGE为GGT-II检测提供了一种简单且可重现的方法。将GGT-II与AFU或AFP联合测定对肝癌的诊断显示出优异的敏感性和特异性。

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