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Jinwei Tang modulates HDAC2 expression in a rat model of COPD

机译：金伟棠调节COPD大鼠模型中HDAC2的表达

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The aim of the present study was to investigate the effect of a Traditional Chinese Herbal Medicine (TCHM), named Jinwei Tang on histone deacetylase 2 (HDAC2) and its role in the regulation of corticosteroid resistance in a rat model of chronic obstructive pulmonary disease (COPD). Male Wistar rats were divided into five groups (each n=10): COPD group, established by the intratracheal instillation of lipopolysaccharide and passive smoke exposure, and control, budesonide, theophylline + budesonide and Jinwei Tang + budesonide groups. Lung function was measured, lung tissue histopathology was examined and HDAC2 expression in the lung was assessed by immunohistochemistry. In addition, protein levels of interleukin-8 (IL-8), tumor necrosis factor (TNF)-α and HDAC2 in lung homogenate were quantified by ELISA. The rat COPD model exhibited alterations of the ratio of forced expiratory volume in 0.2 sec (FEV0.2) to the forced vital capacity, FEV0.2, dynamic compliance and airway resistance. HDAC2 expression was markedly reduced in the lung tissue of the COPD group compared with the control group, and treatment with Jinwei Tang + budesonide or theophylline + budesonide resulted in significant attenuation of the reduction of HDAC2 expression in the lungs (P<0.05). However, treatment with budesonide alone did not significantly alter HDAC2 expression. In the Jinwei Tang + budesonide and theophylline + budesonide groups, IL-8 and TNF-α expression was significantly decreased (P<0.05) and the HDAC2 level increased (P<0.05) compared with that in the COPD group. In conclusion, Jinwei Tang modulates airway inflammation and may enhance the anti-inflammatory effect of glucocorticoid through the upregulation of HADC2 expression in a rat model of COPD.
机译：本研究的目的是研究一种名为金津汤的中草药对组蛋白脱乙酰基酶2（HDAC2）的作用及其在慢性阻塞性肺疾病大鼠模型中对糖皮质激素抵抗的调节作用（ COPD）。将雄性Wistar大鼠分为5组（每组n = 10）：COPD组（通过气管内脂多糖滴注和被动吸烟暴露建立）和对照组，布地奈德，茶碱+布地奈德和金味汤+布地奈德组。测量肺功能，检查肺组织病理学，并通过免疫组织化学评估肺中HDAC2表达。此外，通过ELISA定量了肺匀浆中白细胞介素8（IL-8），肿瘤坏死因子（TNF）-α和HDAC2的蛋白水平。大鼠COPD模型表现出0.2秒的呼气量（FEV0.2）与强制肺活量，FEV0.2，动态顺应性和气道阻力的比率的变化。与对照组相比，COPD组肺组织中HDAC2表达明显降低，金味汤+布地奈德或茶碱+布地奈德治疗显着减弱了肺中HDAC2表达的降低（P <0.05）。但是，仅用布地奈德治疗不会显着改变HDAC2表达。与COPD组相比，金味汤+布地奈德和茶碱+布地奈德组的IL-8和TNF-α表达明显降低（P <0.05），HDAC2水平升高（P <0.05）。总之，金味汤可调节COPD大鼠模型中HADC2表达，从而调节气道炎症并可能增强糖皮质激素的抗炎作用。

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