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An automated system to mount cryo-cooled protein crystals on a synchrotron beam line using compact sample cassettes and a small-scale robot

机译：使用紧凑的样品盒和小型机器人将自动冷却的蛋白晶体安装在同步加速器光束线上的自动化系统

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摘要

An automated system for mounting and dismounting pre-frozen crystals has been implemented at the Stanford Synchrotron Radiation Laboratory (SSRL). It is based on a small industrial robot and compact cylindrical cassettes, each holding up to 96 crystals mounted on Hampton Research sample pins. For easy shipping and storage, the cassette fits inside several popular dry-shippers and long-term storage Dewars. A dispensing Dewar holds up to three cassettes in liquid nitrogen adjacent to the beam line goniometer. The robot uses a permanent magnet tool to extract samples from, and insert samples into a cassette, and a cryo-tong tool to transfer them to and from the beam line goniometer. The system is simple, with few moving parts, reliable in operation and convenient to use.

机译：斯坦福同步辐射实验室（SSRL）已实现了用于安装和拆卸预冷冻晶体的自动化系统。它基于一个小型工业机器人和紧凑的圆柱形盒式磁带，每个盒式磁盘最多可容纳96个安装在Hampton研究样品针上的晶体。为了便于运输和存放，该录像带可放入几种流行的干货和长期存放的杜瓦瓶中。分配杜瓦瓶（Dewar）在靠近束线测角仪的液氮中最多容纳三个盒式磁带。机器人使用永久磁铁工具从样本中提取样本并将样本插入盒中，并使用低温钳工具将样本转移到光束线测角仪中或从波束测角仪中转移出来。该系统简单，运动部件少，运行可靠，使用方便。

著录项

期刊名称 Journal of Applied Crystallography
作者
Aina E. Cohen; Paul J. Ellis; Mitchell D. Miller; Ashley M. Deacon; R. Paul Phizackerley;
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年(卷),期 -1(35),6
年度 -1
页码 720–726
总页数 18
原文格式 PDF
正文语种
中图分类生化遗传学;生化药理学;
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专利

1. An automated system to mount cryo-cooled protein crystals on a synchrotron beamline, using compact sample cassettes and a small-scale robot [J] . Cohen AE., Ellis PJ., Miller MD., Journal of Applied Crystallography . 2002,第6期

机译：使用紧凑的样品盒和小型机器人将自动冷却的蛋白晶体安装在同步加速器光束线上的自动化系统
2. Automated sample mounting and alignment system for biological crystallography at a synchrotron source [J] . Snell G, Cork C, Nordmeyer R, Structure . 2004,第4期

机译：用于同步加速器源的生物晶体学的自动样品安装和对准系统
3. Crystal sample pins and a storage cassette system compatible with the protein crystallography beamlines at both the Photon Factory and SPring-8 [J] . Fujihashi M., Hiraki M., Ueno G., Journal of Applied Crystallography . 2012,第6期

机译：在Photon Factory和SPring-8上，与蛋白质晶体学射线束兼容的晶体样品针和一个存储盒系统
4. Two-Stage Robotic Crystal Mounting of Protein Crystals for X-Ray Data Collection [C] . Atanas Georgiev, Peter K. Allen International Conference on Automation Science and Engineering . 2008

机译：用于X射线数据收集的蛋白质晶体的两级机器人晶体安装
5. Defects Analysis of Wide Bandgap Semiconductor Single Crystals via Synchrotron White Beam X-ray Topography [D] . Sun, Shun 2013

机译：同步加速器白束X射线形貌分析宽带隙半导体单晶的缺陷
6. The Stanford Automated Mounter: pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines [O] . Silvia Russi, Jinhu Song, Scott E. McPhillips, -1

机译：斯坦福自动化贴片机：在SSRL大分子结晶学流水线上提高样品交换的极限
7. An automated system to mount cryo-cooled protein crystals on a synchrotron beamline, using compact sample cassettes and a small-scale robot [O] . Aina E. Cohen, Paul J. Ellis, Mitchell D. Miller, 2002

机译：使用紧凑型样品盒和小规模机器人将冷冻冷却蛋白质晶体安装在同步纸束线上的自动化系统
8. Cluster Ion Beam Studies of Fundamental Dynamics Related to Physical Deposition; Kinematic Sample Mounting System for Accurate Positioning of Transferrable Samples; Sintering, Oxidation, and Chemical Properties of Size- Selected Nickel Clusters on TiO2 (1 [R] . Anderson, S. L., Lapicki, A., Boyd, K. J., 2002

机译：聚类离子束研究与物理沉积相关的基本动力学;可调样品安装系统，用于准确定位可转印样品; TiO2上尺寸选择的镍团簇的烧结，氧化和化学性质（1

An automated system to mount cryo-cooled protein crystals on a synchrotron beam line using compact sample cassettes and a small-scale robot

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