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Crepuscular Behavioral Variation and Profiling of Opsin Genes in Anopheles gambiae and Anopheles stephensi (Diptera: Culicidae)

机译:冈比亚按蚊和斯蒂芬按蚊(Diptera:Culicidae)的夜行行为变异和视蛋白基因谱分析

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摘要

We understand little about photopreference and the molecular mechanisms governing vision-dependent behavior in vector mosquitoes. Investigations of the influence of photopreference on adult mosquito behaviors such as endophagy and exophagy and endophily and exophily will enhance our ability to develop and deploy vector-targeted interventions and monitoring techniques. Our laboratory-based analyses have revealed that crepuscular period photopreference differs between An. gambiae and An. stephensi. We employed qRT-PCR to assess crepuscular transcriptional expression patterns of long wavelength-, short wavelength-, and ultraviolet wavelength-sensing opsins (i.e., rhodopsin-class G-protein coupled receptors) in An. gambiae and in An. stephensi. Transcript levels do not exhibit consistent differences between species across diurnal cycles, indicating that differences in transcript abundances within this gene set are not correlated with these behavioral differences. Using developmentally staged and gender-specific RNAseq data sets in An. gambiae, we show that long wavelength-sensing opsins are expressed in two different patterns (one set expressed during larval stages, and one set expressed during adult stages), while short wavelength- and ultraviolet wavelength-sensing opsins exhibit increased expression during adult stages. Genomic organization of An. gambiae opsins suggests paralogous gene expansion of long wavelength-sensing opsins in comparison with An. stephensi. We speculate that this difference in gene number may contribute to variation between these species in photopreference behavior (e.g., visual sensitivity).
机译:我们对光敏性和控制矢量蚊子中视觉依赖性行为的分子机制了解甚少。研究光敏性对成年蚊子行为的影响,例如内吞和外吞以及内吸和外出嗜热,将增强我们开发和部署针对载体的干预措施和监测技术的能力。我们基于实验室的分析表明,An之间的闭眼期光偏爱有所不同。冈比亚和安。史蒂芬斯。我们使用qRT-PCR来评估An中长波长,短波长和紫外线波长感知视蛋白(即视紫红质类G蛋白偶联受体)的黄昏转录表达模式。冈比亚和安史蒂芬斯。在整个昼夜周期中,转录本水平并未在物种之间表现出一致的差异,这表明该基因集中的转录本丰度差异与这些行为差异不相关。在An。中使用发育阶段的和特定性别的RNAseq数据集。冈比亚,我们显示了长波长敏感视蛋白以两种不同的模式表达(一组在幼虫阶段表达,一组在成年阶段表达),而短波和紫外波长敏感视蛋白在成人阶段表现出增加的表达。 An。的基因组组织。冈比亚视蛋白提示与An相比,长波长感应视蛋白的同源基因扩展。史蒂芬斯。我们推测,这种基因数量上的差异可能会导致这些物种之间在光偏爱行为(例如视觉敏感性)方面的差异。

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