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首页> 美国卫生研究院文献>Journal of Ocular Pharmacology and Therapeutics >Impact of Flow Rate Collection Devices and Extraction Methods on Tear Concentrations Following Oral Administration of Doxycycline in Dogs and Cats
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Impact of Flow Rate Collection Devices and Extraction Methods on Tear Concentrations Following Oral Administration of Doxycycline in Dogs and Cats

机译：狗猫口服强力霉素后流速收集装置和提取方法对泪液浓度的影响

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>Purpose: Compare the precision of doxycycline quantification in tear fluid collected with either Schirmer strips or polyvinyl acetal (PVA) sponges following oral drug administration.>Methods: Three dogs and 3 cats were administered doxycycline orally at a dose of 4.2–5 mg/kg every 12 h for 6 consecutive days. At day 5 and 6, blood and tear fluid were sampled to capture doxycycline trough and maximal concentrations. Tear fluid was collected 3 times (spaced 10 min apart) at each session with the absorbent material placed in the lower conjunctival fornix until the 20-mm mark was reached (Schirmer strip, one eye) or for 1 min (PVA sponge, other eye). Tear extraction was performed with either centrifugation or elution in methanol. Doxycycline concentrations were measured with liquid chromatography–mass spectrometry. Low (100 ng/mL) and high (1,000 ng/mL) tear concentrations measured in vivo were spiked into each absorbent material in vitro to evaluate percentage drug recovery.>Results: After oral administration of doxycycline, the drug reached the tear compartment at concentrations of 45.1–900.7 ng/mL in cats and 45.4–632.0 ng/mL in dogs, representing a tear-to-serum ratio of 12% and 16%, respectively. Doxycycline tear concentrations were significantly more precise when tear collection was performed with Schirmer strips rather than PVA sponges (P = 0.007), but were not correlated with tear flow rate. In vitro doxycycline recovery was poor to moderate (<75%).>Conclusions: Schirmer strips represent a good option for lacrimal doxycycline quantification, although the collection and subsequent extraction have to be optimized to improve drug recovery.
机译：>目的：比较口服药物给药后用Schirmer条或聚乙烯醇缩醛（PVA）海绵收集的泪液中强力霉素定量的准确性。>方法：连续6天每12h口服强力霉素4.2–5μmg / kg。在第5天和第6天，抽取血液和眼泪液以采集多西环素谷和最大浓度。每次将泪液收集3次（间隔10分钟），将吸收性材料置于结膜下穹for下直至达到20毫米标记（Schirmer条，一只眼睛）或1分钟（PVA海绵，另一只眼睛） ）。通过离心或在甲醇中洗脱进行眼泪提取。用液相色谱-质谱法测定强力霉素的浓度。将体内测得的低（100 ng / mL）和高（1,000 ng / mL）泪液浓度加标到每种吸收剂材料中，以评估药物回收率。>结果：口服强力霉素后，在猫中，该药物以45.1–900.7ngng / mL的浓度到达猫的泪液腔室；在狗中，其浓度为45.4–632.0ngng / mL，到达眼泪液的比例分别为12％和16％。用Schirmer试纸条而不是PVA海绵进行泪液收集时，强力霉素的泪液浓度明显更精确（P = 0.007），但与泪液流速无关。体外强力霉素的回收率差到中度（<75％）。>结论： Schirmer胶条是泪道强力霉素定量的一个很好的选择，尽管必须优化收集和随后的提取以提高药物回收率。

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