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首页> 美国卫生研究院文献>Journal of Experimental Botany >Foliar application of zinc sulphate and zinc EDTA to wheat leaves: differences in mobility distribution and speciation
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Foliar application of zinc sulphate and zinc EDTA to wheat leaves: differences in mobility distribution and speciation

机译：叶面喷施硫酸锌和EDTA锌在小麦叶片上的流动性分布和形态差异

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Foliar application of zinc (Zn) to crops is an effective way to increase the grain concentration of Zn. However, the development of more efficient foliar Zn fertilizers is limited by a lack of knowledge regarding the distribution, mobility, and speciation of Zn in leaves once it is taken up by the plant. We performed an experiment using radiolabelled Zn (65Zn), and in situ time-resolved elemental imaging using synchrotron X-ray fluorescence microscopy (XFM), to investigate the behaviour of two commonly used Zn foliar fertilizers (Zn sulphate and ZnEDTA) in wheat (Triticum aestivum) leaves. Both experiments showed that Zn had limited mobility in leaves, moving <25 mm from the application point after 24 h. Although limited, the translocation of Zn occurred quickly for both treatments; moving more between 3 h and 12 h after application than between 12 h and 24 h. Speciation analysis using synchrotron-based X-ray absorption near-edge structure (XANES) showed that ZnEDTA was in fact taken up in chelated form and not as ionic Zn (Zn2+). The XANES data also showed that Zn, from both treatments, was then complexed by ligands in the leaf (e.g. phytate and citrate), potentially in response to localized Zn toxicity. The results of the present study provide important insights into the behaviour of commonly used foliar-applied Zn fertilizers, and can be used to optimize current fertilization strategies and contribute to the development of more efficient foliar Zn fertilizers.
机译：叶面喷施锌（Zn）是增加锌颗粒浓度的有效方法。但是，由于对植物吸收锌后叶片中锌的分布，迁移率和形态的认识不足，限制了更高效的叶面锌肥料的开发。我们使用放射性标记的Zn（ 65 Zn）进行了实验，并使用同步加速器X射线荧光显微镜（XFM）进行了原位时间分辨元素成像，以研究两种常用的Zn叶面肥料（小麦（Triticum aestivum）叶片中的硫酸锌和ZnEDTA）。两项实验均表明，锌在叶片中的迁移率有限，在24小时后距施药点<25 mm。尽管有限，但两种处理中锌的转运都很快。施用后3小时至12小时之间的移动比12小时至24小时之间的移动更多。使用基于同步加速器的X射线吸收近边缘结构（XANES）进行的形态分析表明，ZnEDTA实际上以螯合形式吸收，而不是离子形式的Zn（Zn 2 + ）吸收。 XANES数据还表明，两种处理中的锌随后都被叶中的配体（例如植酸盐和柠檬酸盐）复合，这可能是由于局部Zn毒性所致。本研究的结果为常用叶面施用的锌肥的行为提供了重要的见解，并可用于优化当前的施肥策略并有助于开发更有效的叶面锌肥。

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