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首页> 美国卫生研究院文献>The Journal of Molecular Diagnostics : JMD >Direct Detection of Staphylococcus aureus from Adult and Neonate Nasal Swab Specimens Using Real-Time Polymerase Chain Reaction
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Direct Detection of Staphylococcus aureus from Adult and Neonate Nasal Swab Specimens Using Real-Time Polymerase Chain Reaction

机译：实时聚合酶链反应从成人和新生儿鼻拭子标本中直接检测金黄色葡萄球菌

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摘要

Nasal carriage of Staphylococcus aureus is considered a source of subsequent infection in health care settings. Utilization of real-time polymerase chain reaction (PCR) for detection of S. aureus has the potential to dramatically affect infection control practice by rapidly identifying S. aureus-colonized patients. We developed and validated the use of real-time PCR for detection of S. aureus colonization in two patient populations. Paired nasal swabs were collected from 299 neonates and from 151 adult patients at Evanston Hospital. One swab was used for culture and the other placed into a bacterial lysis solution containing achromopeptidase. The DNA liberated was used as the template for real-time PCR with primers for the femA gene. SYBR Green was used for amplicon detection. In the neonatal population the sensitivity, specificity, predictive value positive and predictive value negative for culture and PCR was 92% versus 96%, 100% versus 100%, 100% versus 100%, and 98% versus 99%, respectively. In the adults the results were 90% versus 100%, 100% versus 98%, 100% versus 96%, and 95% versus 100%, respectively. Real-time PCR was able to detect S. aureus in 2 hours compared to 1 to 4 days for culture and provided sensitivity equal to or greater than culture.
机译：在医疗机构中，金黄色葡萄球菌的鼻腔运输被认为是随后感染的来源。利用实时聚合酶链反应（PCR）检测金黄色葡萄球菌有潜力通过快速识别金黄色葡萄球菌定植的患者来显着影响感染控制实践。我们开发并验证了实时PCR在两个患者群体中检测金黄色葡萄球菌定植的用途。在埃文斯顿医院从299名新生儿和151名成年患者中收集了成对的鼻拭子。一根拭子用于培养，另一根拭子放入含有染色体肽酶的细菌裂解液中。释放的DNA用作femA基因引物进行实时PCR的模板。 SYBR Green用于扩增子检测。在新生儿人群中，培养和PCR的敏感性，特异性，阳性预测值和阴性预测值分别为92％对96％，100％对100％，100％对100％和98％对99％。在成年人中，结果分别为90％对100％，100％对98％，100％对96％和95％对100％。与培养1至4天相比，实时PCR能够在2小时内检测出金黄色葡萄球菌，并提供等于或大于培养的灵敏度。

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