首页> 美国卫生研究院文献>Journal of Experimental Botany >Up-regulating the abscisic acid inactivation gene ZmABA8ox1b contributes to seed germination heterosis by promoting cell expansion
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Up-regulating the abscisic acid inactivation gene ZmABA8ox1b contributes to seed germination heterosis by promoting cell expansion

机译:上脱落酸失活基因ZmABA8ox1b的上调通过促进细胞扩增促进种子萌发杂种优势

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摘要

Heterosis has been widely used in agriculture, but the underlying molecular principles are still largely unknown. During seed germination, we observed that maize (Zea mays) hybrid B73/Mo17 was less sensitive than its parental inbred lines to exogenous abscisic acid (ABA), and endogenous ABA content in hybrid embryos decreased more rapidly than in the parental inbred lines. ZmABA8ox1b, an ABA inactivation gene, was consistently more highly up-regulated in hybrid B73/Mo17 than in its parental inbred lines at early stages of seed germination. Moreover, ectopic expression of ZmABA8ox1b obviously promoted seed germination in Arabidopsis. Remarkably, microscopic observation revealed that cell expansion played a major role in the ABA-mediated maize seed germination heterosis, which could be attributed to the altered expression of cell wall-related genes.
机译:杂种优势已在农业中得到广泛应用,但是基本的分子原理仍然未知。在种子发芽过程中,我们观察到玉米(Zea mays)杂种B73 / Mo17对外源脱落酸(ABA)的敏感性低于其亲本近交系,并且杂种胚中内源ABA的含量下降的速度比亲本近交系更快。 ZmABA8ox1b是一种ABA失活基因,在种子发芽的早期,在杂种B73 / Mo17中始终比在其亲本近交系中上调程度更高。此外,异位表达ZmABA8ox1b明显促进拟南芥种子的萌发。值得注意的是,显微镜观察显示,细胞膨胀在ABA介导的玉米种子萌发杂种优势中起主要作用,这可能归因于细胞壁相关基因表达的改变。

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