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首页> 美国卫生研究院文献>Oncology Letters >Lily extracts inhibit the proliferation of gastric carcinoma SGC-7901 cells by affecting cell cycle progression and apoptosis via the upregulation of caspase-3 and Fas proteins and the downregulation of FasL protein
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Lily extracts inhibit the proliferation of gastric carcinoma SGC-7901 cells by affecting cell cycle progression and apoptosis via the upregulation of caspase-3 and Fas proteins and the downregulation of FasL protein

机译：百合提取物通过上调caspase-3和Fas蛋白的表达并下调FasL蛋白的表达影响细胞周期进程和凋亡从而抑制SGC-7901细胞的增殖。

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The present study aimed to investigate the effect of alkaloids and carbinol extracts from lily on the proliferation of SGC-7901 cells, as well as the underlying mechanism. SGC-7901 cells were incubated with different concentrations of alkaloid or carbinol extracts for 24, 48 or 72 h. MTT assays were used to measure the inhibition rate of SGC-7901 cell proliferation. Inverted phase contrast and fluorescence microscopy was used to observe morphological changes of SGC-7901 cells. Flow cytometry was employed to detect cell cycle progression and apoptosis rates of SGC-7901 cells. Western blotting was performed to measure the expression of caspase-3, Fas and Fas ligand (FasL) proteins in SGC-7901 cells. The inhibition rate of SGC-7901 cell proliferation was significantly enhanced with increasing drug concentrations and time elapsed. Treatment with alkaloid or carbinol extracts deteriorated the morphology of SGC-7901 cells in a dose-dependent manner. Alkaloid and carbinol extracts arrested SGC-7901 cells in the G2/M phase, and induced apoptosis in a dose-dependent manner. Alkaloid and carbinol extracts enhanced caspase-3, and Fas expression, but reduced FasL expression in SGC-7901 cells. The present study demonstrated that alkaloids and carbinol extracts from lily inhibited the proliferation of gastric carcinoma SGC-7901 cells by arresting cells in the G2/M phase. The upregulation of caspase-3 and Fas proteins, and the downregulation of FasL protein may be an important mechanism for the induction of SGC-7901 cell apoptosis.
机译：本研究旨在研究百合中生物碱和甲醇提取物对SGC-7901细胞增殖的影响及其潜在机制。将SGC-7901细胞与不同浓度的生物碱或甲醇提取物孵育24、48或72小时。使用MTT测定法测量SGC-7901细胞增殖的抑制率。倒置相差和荧光显微镜观察SGC-7901细胞的形态变化。流式细胞仪用于检测SGC-7901细胞的细胞周期进程和凋亡率。进行了蛋白质印迹法，以测量SGC-7901细胞中caspase-3，Fas和FasL配体（FasL）蛋白的表达。随着药物浓度和时间的增加，SGC-7901细胞增殖的抑制率显着提高。生物碱或甲醇提取物的处理以剂量依赖的方式恶化了SGC-7901细胞的形态。生物碱和甲醇提取物使SGC-7901细胞停滞在G2 / M期，并以剂量​​依赖性方式诱导细胞凋亡。生物碱和甲醇提取物增强了SGC-7901细胞中的caspase-3和Fas表达，但降低了FasL表达。本研究表明，百合中的生物碱和甲醇提取物通过将细胞阻滞在G2 / M期来抑制胃癌SGC-7901细胞的增殖。 caspase-3和Fas蛋白的上调以及FasL蛋白的下调可能是诱导SGC-7901细胞凋亡的重要机制。

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