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首页> 美国卫生研究院文献>Molecular Medicine Reports >Essential oil of Artemisia vestita exhibits potent in vitro and in vivo antibacterial activity: Investigation of the effect of oil on biofilm formation leakage of potassium ions and survival curve measurement
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Essential oil of Artemisia vestita exhibits potent in vitro and in vivo antibacterial activity: Investigation of the effect of oil on biofilm formation leakage of potassium ions and survival curve measurement

机译：蒿蒿精油在体外和体内均具有有效的抗菌活性：研究精油对生物膜形成钾离子泄漏和存活曲线测量的影响

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摘要

The aim of the present study was to investigate the chemical composition of the essential oil of Artemisia vestita and to determine the antibacterial activity of the essential oil and its two major components, grandisol and 1,8-cineole, against certain respiratory infection-causing bacterial strains, in vitro and in vivo. The chemical composition of the essential oil was analyzed using gas chromatography-mass spectrometry. A micro-well dilution method was used to determine the minimum inhibition concentration (MIC) values of the essential oil and its major constituents. A model of Streptococcus pyogenes infection in mice was used to determine its in vivo activities. Lung and blood samples were obtained to assess bacterial cell counts. Toxicity evaluation of the essential oil and its components was completed by performing biochemical analysis of the serum, particularly monitoring aspartate transaminase, alanine transaminase, urea and creatinine. The essential oil exhibited potent antibacterial activity, whereas the two major constituents were less potent. The essential oil exhibited MIC values between 20 and 80 μg/ml, while the values of the two constituents were between 130 and 200 μg/ml. Scanning electron microscopy results demonstrated that the essential oil inhibited biofilm formation and altered its architecture. Survival curves indicated that the essential oil led to a reduction in the viability of different bacteria. The essential oil also induced significant leakage of potassium ions from S. pyogenes. The essential oil (100 μg/mouse) and grandisol (135 μg/mouse) significantly reduced the number of viable bacterial cells in the lungs (P<0.01). However, intake of 100 μg/mouse of essential oil or grandisol 135 μg/mouse once or twice each day for 9 days did not produce any toxic effects in the mice. In conclusion, the in vitro and in vivo results suggested that the essential oil of A. vestita and one of its major constituents, grandisol, can significantly inhibit the growth of different bacterial strains.
机译：本研究的目的是调查蒿蒿精油的化学成分，并确定精油及其两个主要成分大扁豆酚和1,8-桉树脑对某些引起呼吸道感染的细菌的抗菌活性。体内和体外菌株。使用气相色谱-质谱法分析精油的化学组成。使用微孔稀释法确定精油及其主要成分的最小抑菌浓度（MIC）值。用小鼠化脓性链球菌感染模型来确定其体内活性。获得肺和血液样品以评估细菌细胞计数。通过对血清进行生化分析，特别是监测天冬氨酸转氨酶，丙氨酸转氨酶，尿素和肌酐，可以完成精油及其成分的毒性评估。精油表现出有效的抗菌活性，而两种主要成分的效力较弱。精油的MIC值在20至80μg/ ml之间，而两种成分的MIC在130至200μg/ ml之间。扫描电子显微镜结果表明，精油抑制了生物膜的形成并改变了其结构。存活曲线表明，精油导致不同细菌的活力降低。精油还引起化脓性链球菌中钾离子的大量泄漏。精油（每只小鼠100微克）和金刚烷醇（每只小鼠135微克）显着减少了肺中活菌的数量（P <0.01）。但是，每天一次或两次每天摄入100μg/小鼠精油或randisol 135μg/小鼠，连续9天对小鼠没有产生任何毒性作用。总之，体外和体内结果表明，A.vesitaa香精油及其主要成分之一Grandisol可以显着抑制不同细菌菌株的生长。

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