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首页> 美国卫生研究院文献>SpringerPlus >Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry
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Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry

机译：同时测定黄芪中六种活性代谢物的含量。串联质谱法在盐胁迫下超压液相色谱

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摘要

Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao (A. mongholicus, family Leguminosae) is one of the most important traditional Chinese herbs because it contains lots of bioactive metabolites, which have beneficial and pharmacological effects on health. Simultaneously, it has been proved to be a salt-tolerant plant—one of the potential species to control the soil salinization. Therefore, a sensitive and specific ultra-pressure liquid chromatography coupled with tandem mass spectrometric (UPLC-MS/MS) method was developed and validated for the simultaneous determination of six main bioactive metabolites, astragaloside IV, cycloastragenol, calycosin-7-O-β-d-glucoside, calycosin, ononin and formononetin in different organs of A. mongholicus. The detection was accomplished by multiple-reaction monitoring (MRM) scanning via electrospray ionization source operating in the positive ionization mode. Calibration curves offered linear ranges of two orders of magnitude with R2 > 0.99. The method was fully validated for the linearity, intra-day and inter day precisions, accuracy, recovery, matrix effect and stability. Then this method was successfully applied to detect the content of major bioactive metabolites in different plant organs of A. mongholicus under salt stress. Significant variations in the content of six bioactive metabolites were observed after been processed by different levels of salinity in different part of plant. The results support for further exploration of the salt-tolerant mechanisms in A. mongholicus and its possibility as the species that control the soil salinization. Meanwhile, we established a UPLC-MS/MS assay of the trace components in seedling of A. mongholicus in this study.
机译：黄芪。变种gh（A. mongholicus，豆科家族）是最重要的传统中草药之一，因为它含有大量的生物活性代谢物，对健康具有有益和药理作用。同时，它已被证明是一种耐盐植物，是控制土壤盐碱化的潜在物种之一。因此，开发了灵敏且特异的超高压液相色谱-串联质谱（UPLC-MS / MS）方法，并验证了该方法可同时测定六种主要生物活性代谢物：黄芪甲苷IV，环黄芪醇，calycosin-7-O-β -d-葡萄糖苷，calycosin，ononin和formononetin在蒙古曲霉的不同器官中该检测是通过以正电离模式运行的电喷雾电离源通过多反应监测（MRM）扫描完成的。校准曲线提供两个数量级的线性范围，R 2

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