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首页> 美国卫生研究院文献>Tissue Engineering. Part A >Tissue-Engineered Cartilaginous Constructs for the Treatment of Caprine Cartilage Defects Including Distribution of Laminin and Type IV Collagen
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Tissue-Engineered Cartilaginous Constructs for the Treatment of Caprine Cartilage Defects Including Distribution of Laminin and Type IV Collagen

机译：组织工程化的软骨构建体用于治疗鸡软骨缺损包括层粘连蛋白和IV型胶原蛋白的分布

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The purpose of this study was the immunohistochemical evaluation of (1) cartilage tissue-engineered constructs; and (2) the tissue filling cartilage defects in a goat model into which the constructs were implanted, particularly for the presence of the basement membrane molecules, laminin and type IV collagen. Basement membrane molecules are localized to the pericellular matrix in normal adult articular cartilage, but have not been examined in tissue-engineered constructs cultured in vitro or in tissue filling cartilage defects into which the constructs were implanted. Cartilaginous constructs were engineered in vitro using caprine chondrocyte-seeded type II collagen scaffolds. Autologous constructs were implanted into 4-mm-diameter defects created to the tidemark in the trochlear groove in the knee joints of skeletally mature goats. Eight weeks after implantation, the animals were sacrificed. Constructs underwent immunohistochemical and histomorphometric evaluation. Widespread staining for the two basement membrane molecules was observed throughout the extracellular matrix of in vitro and in vivo samples in a distribution unlike that previously reported for cartilage. At sacrifice, 70% of the defect site was filled with reparative tissue, which consisted largely of fibrous tissue and some fibrocartilage, with over 70% of the reparative tissue bonded to the adjacent host tissue. A novel finding of this study was the observation of laminin and type IV collagen in in vitro engineered cartilaginous constructs and in vivo cartilage repair samples from defects into which the constructs were implanted, as well as in normal caprine articular cartilage. Future work is needed to elucidate the role of basement membrane molecules during cartilage repair and regeneration.
机译：本研究的目的是对（1）软骨组织工程构建物进行免疫组织化学评估； （2）植入构建体的山羊模型中的组织填充软骨缺损，特别是对于存在基底膜分子，层粘连蛋白和IV型胶原的情况。基底膜分子位于正常成人关节软骨中的细胞周围基质中，但尚未在体外培养的组织工程构造中或植入该构造的组织填充软骨缺损中进行检查。使用山羊软骨播种的II型胶原蛋白支架在体外对软骨构建体进行了工程改造。将自体构建体植入直径为4毫米的缺陷中，该缺陷产生到骨骼成熟山羊的膝关节滑车槽中的潮汐标记。植入后八周，处死动物。对构建体进行免疫组织化学和组织形态计量学评估。与以前报道的软骨分布不同，在体外和体内样品的整个细胞外基质中都观察到了两个基膜分子的广泛染色。处死时，70％的缺损部位充满了修复组织，该组织主要由纤维组织和一些纤维软骨组成，超过70％的修复组织与相邻的宿主组织结合。这项研究的新发现是在体外工程化的软骨构建体和植入该构建体的缺陷的体内软骨修复样品以及正常的山羊关节软骨中观察层粘连蛋白和IV型胶原。需要进一步的工作阐明基底膜分子在软骨修复和再生中的作用。

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