首页> 美国卫生研究院文献>SpringerPlus >Application of direct PCR in rapid rDNA ITS haplotype determination of the hyperparasitic fungus Sphaeropsis visci (Botryosphaeriaceae)

【2h】

Application of direct PCR in rapid rDNA ITS haplotype determination of the hyperparasitic fungus Sphaeropsis visci (Botryosphaeriaceae)

机译：直接PCR技术在快速检测超寄生真菌黏链霉菌（Botryosphaeriaceae）的rDNA ITS单倍型中的应用

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

BackgroundThe plant pathogenic fungus, Sphaeropsis visci a dark-spored species of Botryosphaeriaceae, which causes the leaf spot disease of the European mistletoe (Viscum album). This species seems to have potential as a tool for biological control of the hemiparasite. For the rapid detection of S. visci haplotypes we tested a direct PCR assay without prior DNA purification. This approach was based on a polymerase enzyme from the crenarchaeon Sulfolobus solfataricus engineered by fusion protein technology, which linked the polymerase domain to a sequence non-specific DNA binding protein (Sso7d).

机译：背景植物病原性真菌Sphaeropsis visci是一种深色孢子菌，其引起欧洲槲寄生（Viscum album）的叶斑病。该物种似乎有潜力作为半寄生虫生物防治的工具。为了快速检测粘链链球菌单倍型，我们测试了直接的PCR检测方法，无需事先进行DNA纯化。该方法基于融合蛋白技术设计的来自crenarchaeon solfolobus solfataricus的聚合酶，该酶将聚合酶结构域连接至序列非特异性DNA结合蛋白（Sso7d）。

著录项

期刊名称 SpringerPlus
作者
Ildikó Varga; Péter Poczai; István Cernák; Jaakko Hyvönen;
展开▼
作者单位

展开▼
年(卷),期 -1(3),-1
年度 -1
页码 569
总页数 9
原文格式 PDF
正文语种
中图分类
关键词
Biological control Botryosphaeriaceae diPCR European mistletoe (Viscum album) Genotyping Polymerase inhibitors Sphaeropsis visci;

机译：生物防治;葡萄孢菌;diPCR;欧洲槲寄生（Viscum album）;基因分型;聚合酶抑制剂;黏菌;

相似文献

外文文献
中文文献
专利

1. Internal transcribed spacer (ITS) evolution in populations of the hyperparasitic European mistletoe pathogen fungus, Sphaeropsis visci (Botryosphaeriaceae): The utility of ITS2 secondary structures [J] . Poczai Peter, Varga Ildiko, Hyvonen Jaakko Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function . 2015,第1期

机译：内转录间隔物（ITS）在超寄生欧洲槲寄生病原真菌，鞘脂蝶（Botryosphaeriaceae）种群中的演变：ITS2二级结构的实用性。
2. OPTIMISATION OF GROWING CONDITIONS OF EUROPEAN MISTLETOE HYPERPARASITIC FUNGUS [PHAEOBOTRYOSPHAERIA VISCI): EFFECT OF DIFFERENT MEDIA AND ANTIBIOTICS [J] . lldikó Varga, Tivadar Baltazar, Milos Pejchal Acta horticulturae et regiotecturae . 2013,第2期

机译：欧洲槲寄生高寄生性真菌[PHAEOBOTRYOSPHAERIA VISCI）生长条件的优化：不同培养基和抗生素的作用
3. Rapid and Direct Detection of Clostridium chauvoei by PCR of the 16S-23S rDNA Spacer Region and Partial 23S rDNA Sequences [J] . Akemi KOJIMA, Kinya YAMAMOTO, Mari NORIMATSU, The Journal of Veterinary Medical Science . 2000,第12期

机译：通过16S-23S rDNA间隔区和23S rDNA的部分序列的PCR快速直接检测破伤风梭菌
4. Rapid detection of Liberobacter asiaticum in citrus by PCR amplification of 16S rDNA and ribosomal protein [C] . Wang Zhong-kang, Timothy C.Gast, J.M.Molin Asian conference on plant pathology . 2000

机译：PCR扩增16S rDNA和核糖体蛋白的PCR扩增快速检测柑橘体亚胺菌
5. The application of real -time PCR to investigate the effect of the arbuscular mycorrhizal fungus Glomus intraradices on the plant pathogen Fusarium solani f. sp. phaseoli. [D] . Filion, Martin. 2003

机译：实时PCR技术在研究丛枝菌根真菌Glomus intraradices对植物病原枯萎病镰刀菌的影响。 sp。菜豆。
6. Direct haplotype determination by double ARMS: specificity sensitivity and genetic applications. [O] . Y M Lo, P Patel, C R Newton, 1991

机译：通过双ARMS直接测定单倍型：特异性敏感性和遗传应用。
7. Application of direct PCR in rapid rDNA ITS haplotype determination of the hyperparasitic fungus Sphaeropsis visci (Botryosphaeriaceae) [O] . Ildikó Varga, Péter Poczai, István Cernák, 2014

机译：直接PCR技术在快速检测超寄生真菌黏链霉菌（Botryosphaeriaceae）的rDNA ITS单倍型中的应用

Application of direct PCR in rapid rDNA ITS haplotype determination of the hyperparasitic fungus Sphaeropsis visci (Botryosphaeriaceae)

摘要

著录项

相似文献

相关主题

期刊订阅