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A novel Arabidopsis marker line that strongly labels uninucleate microspores and the subsequent male gametophyte development stages

机译:一种新型拟南芥标记物系可强烈标记单核小孢子和随后的雄配子体发育阶段

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摘要

Here is reported a novel histological marker line that strongly and specifically labels the male gametophyte in Arabidopsis flowers, from the uninucleate microspore to the pollination stage. In this marker line, the expression of the UidA and GFP marker genes is driven by the promoter of the At5g17340 Arabidopsis gene, a gene highly expressed in the male gametophyte. Strong GUS histochemical staining is detected in the uninucleate microspores. Strong GUS, and GFP, histological labelling is also detected in the subsequent stages of male gametophyte development. The potential applications of this marker line are discussed.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-2-237) contains supplementary material, which is available to authorized users.
机译:此处报道了一种新颖的组织学标记物系,该标记物从单核小孢子到授粉期强烈而特异性地标记了拟南芥花中的雄配子体。在该标记系中,UidA和GFP标记基因的表达受At5g17340拟南芥基因(该基因在雄配子体中高度表达)的启动子驱动。在单核小孢子中检测到强GUS组织化学染色。在雄配子体发育的后续阶段也检测到了强GUS和GFP的组织学标记。电子补充材料本文的在线版本(doi:10.1186 / 2193-1801-2-237)包含补充材料,授权用户可以使用。

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