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Identification of bacterial communities in sediments of Poyang Lake the largest freshwater lake in China

机译:中国最大的淡水湖泊Po阳湖沉积物中细菌群落的鉴定

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摘要

Bacteria play a vital role in various biogeochemical processes in lacustrine sediment ecosystems. This study is among the first to investigate the spatial distribution patterns of bacterial community composition in the sediments of Poyang Lake, the largest freshwater lake of China. Sediment samples were collected from the main basins and mouths of major rivers that discharge into the Poyang Lake in May 2011. Quantitative PCR assay and pyrosequencing analysis of 16S rRNA genes showed that the bacteria community abundance and compositions of Poyang Lake sediment varied largely among sampling sites. A total of 25 phyla and 68 bacterial orders were distinguished. Burkholderiales, Gallionellales (Beta-proteobacteria), Myxococcales, Desulfuromonadales (Delta-proteobacteria), Sphingobacteriales (Bacteroidetes), Nitrospirales (Nitrospirae), Xanthomonadales (Gamma-proteobacteria) were identified as the major taxa and collectively accounted for over half of annotated sequences. Moreover, correlation analyses suggested that higher loads of total phosphorus and heavy metals (copper, zinc and cadmium) could enhance bacterial abundance in the sediment. Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-2026-7) contains supplementary material, which is available to authorized users.
机译:细菌在湖沉积物生态系统的各种生物地球化学过程中起着至关重要的作用。这项研究是首次调查中国最大的淡水湖泊Po阳湖沉积物中细菌群落组成的空间分布格局。从2011年5月排入discharge阳湖的主要河流主要河口和河口收集沉积物样品。定量PCR分析和16S rRNA基因的焦磷酸测序分析表明,sampling阳湖沉积物的细菌群落丰度和组成在采样点之间存在很大差异。总共区分出25个门和68个细菌。伯克霍尔德氏菌,Gallionellales(β-变形杆菌),粘菌球菌,Desulfuromonadales(δ-变形杆菌),Sphingobacteriales(拟杆菌),Nitrospirales(Nitrospirae),Xanthomonadales(γ-变形杆菌)被认为是主要的分类群,并且被集体认为是主要的分类群。此外,相关分析表明,较高的总磷和重金属(铜,锌和镉)含量可以增加沉积物中细菌的丰度。电子补充材料本文的在线版本(doi:10.1186 / s40064-016-2026-7)包含补充材料,授权用户可以使用。

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