• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Scientific Reports >Microsatellite Borders and Micro-sequence Conservation in Juglans
【2h】

Microsatellite Borders and Micro-sequence Conservation in Juglans

机译：胡格兰的微卫星边界和微序列保护

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Walnuts (Juglans spp.) are economically important nut and timber species with a worldwide distribution. Using the published Persian walnut genome as a reference for the assembly of short reads from six Juglans species and several interspecific hybrids, we identified simple sequence repeats in 12 Juglans nuclear and organellar genomes. The genome-wide distribution and polymorphisms of nuclear and organellar microsatellites (SSRs) for most Juglans genomes have not been previously studied. We compared the frequency of nuclear SSR motifs and their lengths across Juglans, and identified section-specific chloroplast SSR motifs. Primer pairs were designed for more than 60,000 SSR-containing sequences based on alignment against assembled scaffold sequences. Of the >60,000 loci, 39,000 were validated by e-PCR using unique primer pairs. We identified primers containing 100% sequence identity in multiple species. Across species, sequence identity in the SSR-flanking regions was generally low. Although SSRs are common and highly dispersed in the genome, their flanking sequences are conserved at about 90 to 95% identity within Juglans and within species. In a few rare cases, flanking sequences are identical across species of Juglans. This comprehensive report of nuclear and organellar SSRs in Juglans and the generation of validated SSR primers will be a useful resource for future genetic analyses, walnut breeding programs, high-level taxonomic evaluations, and genomic studies in Juglandaceae.
机译：核桃（Juglans spp。）是具有重要经济意义的坚果和木材种类，分布在世界各地。使用已出版的波斯核桃基因组作为参考，以汇编来自6个Juglans物种和几种种间杂种的短读，我们确定了12个Juglans核和细胞体基因组中的简单序列重复。先前尚未研究过大多数Juglans基因组的核和细胞微卫星（SSR）的全基因组分布和多态性。我们比较了核SSR基序的频率及其在胡桃木上的长度，并确定了特定于部分的叶绿体SSR基序。基于与组装的支架序列的比对，针对超过60,000个含SSR的序列设计了引物对。在> 60,000个基因座中，有39,000个通过独特的引物对通过e-PCR验证。我们鉴定了多个物种中包含100％序列同一性的引物。在整个物种中，SSR侧翼区域的序列同一性通常较低。尽管SSR是常见的，并且高度分散在基因组中，但它们的侧翼序列在Juglans和物种内以大约90％到95％的一致性被保守。在极少数情况下，侧翼序列在胡桃属物种之间是相同的。这份有关胡桃岛核和细胞态SSR的综合报告以及经过验证的SSR引物的产生，将为将来的遗传分析，核桃育种计划，高级分类学评估以及胡桃科的基因组研究提供有用的资源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号