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Comparative genomic and functional analyses of four sequenced Bacillus cereus genomes reveal conservation of genes relevant to plant-growth-promoting traits

机译:四个蜡样芽胞杆菌基因组的比较基因组和功能分析揭示了与促进植物生长的性状相关的基因的保守性

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摘要

Some Bacillus strains function as predominant plant-growth-promoting rhizobacteria. Bacillus cereus 905 is a rod-shaped Gram-positive bacterium isolated from wheat rhizosphere and is a rhizobacterium that exhibits significant plant-growth-promoting effects. Species belonging to the genus Bacillus are observed in numerous different habitats. Several papers on B. cereus are related to pathogens that causes food-borne illness and industrial applications. However, genomic analysis of plant-associated B. cereus has yet to be reported. Here, we conducted a genomic analysis comparing strain 905 with three other B. cereus strains and investigate the genomic characteristics and evolution traits of the species in different niches. The genome sizes of four B. cereus strains range from 5.38 M to 6.40 M, and the number of protein-coding genes varies in the four strains. Comparisons of the four B. cereus strains reveal 3,998 core genes. The function of strain-specific genes are related to carbohydrate, amino acid and coenzyme metabolism and transcription. Analysis of single nucleotide polymorphisms (SNPs) indicates local diversification of the four strains. SNPs are unevenly distributed throughout the four genomes, and function interpretation of regions with high SNP density coincides with the function of strain-specific genes. Detailed analysis indicates that certain SNPs contribute to the formation of strain-specific genes. By contrast, genes related to plant-growth-promoting traits are highly conserved. This study shows the genomic differences between four strains from different niches and provides an in-depth understanding of the genome architecture of these species, thus facilitating genetic engineering and agricultural applications in the future.
机译:一些芽孢杆菌属菌株起着促进植物生长的根际细菌的作用。蜡状芽孢杆菌905是从小麦根际分离的杆状革兰氏阳性细菌,并且是表现出显着的促进植物生长作用的根际细菌。在许多不同的生境中观察到属于芽孢杆菌属的物种。关于蜡状芽孢杆菌的几篇论文都涉及引起食源性疾病和工业应用的病原体。但是,尚未报道与植物相关的蜡状芽孢杆菌的基因组分析。在这里,我们进行了基因组分析,将905菌株与其他三个蜡状芽孢杆菌菌株进行了比较,并研究了该物种在不同生态位中的基因组特征和进化特征。四个蜡状芽孢杆菌菌株的基因组大小范围为5.38 M至6.40 M,并且这四个菌株中蛋白质编码基因的数量各不相同。四种蜡状芽孢杆菌菌株的比较显示了3,998个核心基因。菌株特异性基因的功能与碳水化合物,氨基酸以及辅酶的代谢和转录有关。单核苷酸多态性(SNPs)的分析表明这四个菌株的局部多样化。 SNP在四个基因组中分布不均,具有高SNP密度的区域的功能解释与菌株特异性基因的功能一致。详细分析表明某些SNP有助于形成菌株特异性基因。相反,与促进植物生长的性状有关的基因是高度保守的。这项研究显示了来自不同生态位的四种菌株之间的基因组差异,并提供了对这些物种的基因组结构的深入了解,从而促进了基因工程和未来的农业应用。

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