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Chemically monoubiquitinated PEX5 binds to the components of the peroxisomal docking and export machinery

机译：化学单泛素化的PEX5与过氧化物酶体对接和出口机械的组件结合

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摘要

Peroxisomal matrix proteins contain either a peroxisomal targeting sequence 1 (PTS1) or a PTS2 that are recognized by the import receptors PEX5 and PEX7, respectively. PEX5 transports the PTS1 proteins and the PEX7/PTS2 complex to the docking translocation module (DTM) at the peroxisomal membrane. After cargo release PEX5 is monoubiquitinated and extracted from the peroxisomal membrane by the receptor export machinery (REM) comprising PEX26 and the AAA ATPases PEX1 and PEX6. Here, we investigated the protein interactions of monoubiquitinated PEX5 with the docking proteins PEX13, PEX14 and the REM. “Click” chemistry was used to synthesise monoubiquitinated recombinant PEX5. We found that monoubiquitinated PEX5 binds the PEX7/PTS2 complex and restores PTS2 protein import in vivo in ΔPEX5 fibroblasts. In vitro pull-down assays revealed an interaction of recombinant PEX5 and monoubiquitinated PEX5 with PEX13, PEX14 and with the REM components PEX1, PEX6 and PEX26. The interactions with the docking proteins were independent of the PEX5 ubiquitination status whereas the interactions with the REM components were increased when PEX5 is ubiquitinated.
机译：过氧化物酶体基质蛋白包含过氧化物酶体靶向序列1（PTS1）或PTS2，它们分别被输入受体PEX5和PEX7识别。 PEX5将PTS1蛋白和PEX7 / PTS2复合物转运至过氧化物酶体膜的对接转运模块（DTM）。货物释放后，PEX5被单泛素化，并通过包含PEX26和AAA ATPases PEX1和PEX6的受体输出机制（REM）从过氧化物酶体膜中提取出来。在这里，我们研究了单泛素化的PEX5与对接蛋白PEX13，PEX14和REM的蛋白质相互作用。 “点击”化学用于合成单泛素化的重组PEX5。我们发现单泛素化的PEX5结合PEX7 / PTS2复合物，并在ΔPEX5成纤维细胞中恢复PTS2蛋白在体内的导入。体外下拉测定法揭示了重组PEX5和单泛素化PEX5与PEX13，PEX14以及REM成分PEX1，PEX6和PEX26的相互作用。与停靠蛋白的相互作用与PEX5泛素化状态无关，而当PEX5泛素化时，与REM成分的相互作用增加。

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