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Water impacts nutrient dose responses genome-wide to affect crop production

机译:水影响全基因组的养分剂量响应影响作物产量

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摘要

Changes in nutrient dose have dramatic effects on gene expression and development. One outstanding question is whether organisms respond to changes in absolute nutrient amount (moles) vs. its concentration in water (molarity). This question is particularly relevant to plants, as soil drying can alter nutrient concentration, without changing its absolute amount. To compare the effects of amount vs. concentration, we expose rice to a factorial matrix varying the dose of nitrogen (N) and water (W) over a range of combinations, and quantify transcriptome and phenotype responses. Using linear models, we identify distinct dose responses to either N-moles, W-volume, N-molarity (N/W), or their synergistic interaction (N×W). Importantly, genes whose expression patterns are best explained by N-dose and W interactions (N/W or N×W) in seedlings are associated with crop outcomes in replicated field trials. Such N-by-W responsive genes may assist future efforts to develop crops resilient to increasingly arid, low nutrient soils.
机译:营养剂量的变化对基因表达和发育有显着影响。一个悬而未决的问题是生物体是否对绝对养分含量(摩尔)与其在水中的浓度(摩尔)的变化作出反应。这个问题与植物特别相关,因为土壤干燥可以改变养分浓度,而不改变其绝对含量。为了比较数量与浓度的影响,我们将水稻暴露于阶乘矩阵中,该阶乘矩阵在一系列组合中改变氮(N)和水(W)的剂量,并量化转录组和表型响应。使用线性模型,我们确定了对N摩尔,W体积,N摩尔(N / W)或其协同相互作用(N×W)的不同剂量响应。重要的是,在重复田间试验中,其表达模式最能通过幼苗中的N剂量和W相互作用(N / W或N×W)解释的基因与作物结果相关。这样的N-W响应基因可能有助于将来开发对干旱,低养分土壤有弹性的作物。

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