• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Oncology Letters >RNAi targeting of hTERT gene expression induces apoptosis and inhibits the proliferation of lung cancer cells
【2h】

RNAi targeting of hTERT gene expression induces apoptosis and inhibits the proliferation of lung cancer cells

机译：靶向hTERT基因表达的RNAi诱导凋亡并抑制肺癌细胞的增殖

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present study aimed to investigate the effects of RNAi-mediated reduction in human telomerase reverse transcriptase (hTERT) expression on apoptosis and lung cancer cell proliferation. A number of cell lines, including 95D, were used. hTERT mRNA levels were detected, and the RNA concentration was calculated. MTT assay was used to detect the inhibition of cell proliferation. The siRNA with the highest suppression rate, siRNA-1, was transfected into 95D cells at three different concentrations (50, 80 and 100 nmol/l). The levels of hTERT mRNA in cells transfected with 50 nmol/l siRNA-1 were not significantly different from those of the negative control-transfected cells (P>0.05), whereas both 80 and 100 nmol/l siRNA-1 showed significant reductions in hTERT mRNA compared to the negative control cells (P<0.01). hTERT levels in the 80- and 100-nmol/l groups were not significantly different (P>0.05). Compared with the control cells, cells transfected with 50, 80 or 100 nmol/l siRNA-1 showed higher fractions of apoptotic cells 48 h post-transfection (P<0.01), although the apoptotic fraction in cells transfected with 50 nmol/l siRNA-1 was not significantly different compared to that in cells transfected with negative control siRNAs (P>0.05). Moreover, the 80- and 100-nmol/l-transfected cells showed significantly increased apoptotic indices (P<0.01). MTT results indicated a time-dependent inhibition of siRNA-1- transfected cell proliferation starting at 12 h and lasting through 48 h post-transfection; the inhibition was attenuated by 72 h post-transfection. The high levels of hTERT mRNA in all human lung cancer cell lines tested suggest that telomerase plays a role in lung carcinogenesis, and this hypothesis was strengthened by the data showing that the siRNA-mediated reduction in hTERT mRNA caused apoptosis and an inhibition of the proliferation of lung cancer cells.
机译：本研究旨在调查RNAi介导的人类端粒酶逆转录酶（hTERT）表达减少对细胞凋亡和肺癌细胞增殖的影响。使用了许多细胞系，包括95D。检测hTERT mRNA水平，并计算RNA浓度。使用MTT测定法检测细胞增殖的抑制。将抑制率最高的siRNA siRNA-1以三种不同的浓度（50、80和100 nmol / l）转染到95D细胞中。用50 nmol / l siRNA-1转染的细胞中hTERT mRNA的水平与阴性对照转染细胞的hTERT mRNA的水平没有显着差异（P> 0.05），而80和100 nmol / l siRNA-1的HTERT mRNA均显着降低。 hTERT mRNA与阴性对照细胞相比（P <0.01）。 80-nmol / l和100-nmol / l组的hTERT水平无显着差异（P> 0.05）。与对照细胞相比，转染后48 h，用50、80或100 nmol / l siRNA-1转染的细胞显示出更高的凋亡分数（P <0.01），尽管用50 nmol / l siRNA转染的细胞中有凋亡分数。 -1与转染阴性对照siRNA的细胞相比无显着差异（P> 0.05）。此外，转染80-nmol / l和100-nmol / l的细胞显示出明显的凋亡指数（P <0.01）。 MTT结果表明siRNA-1转染的细胞增殖的时间依赖性抑制作用始于12小时，持续至转染后48小时。转染后72小时抑制作用减弱。在所有测试的人类肺癌细胞系中高水平的hTERT mRNA提示端粒酶在肺癌发生过程中起着重要作用，这一数据进一步证实了这一假设，表明siRNA介导的hTERT mRNA的降低会导致细胞凋亡并抑制增殖肺癌细胞。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号