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首页> 美国卫生研究院文献>Scientific Reports >A turn-on endoplasmic reticulum-targeted two-photon fluorescent probe for hydrogen sulfide and bio-imaging applications in living cells tissues and zebrafish
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A turn-on endoplasmic reticulum-targeted two-photon fluorescent probe for hydrogen sulfide and bio-imaging applications in living cells tissues and zebrafish

机译：靶向内质网的双光子荧光探针用于活细胞组织和斑马鱼中的硫化氢和生物成像应用

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As one of the important gas signal molecules, hydrogen sulfide (H2S) is associated with many important physiological processes in living organisms. Organelles, especially endoplasmic reticulum (ER), play a crucial role in the cell metabolism. Accordingly, the detection of H2S in the ER is of high interest. Toward this goal, we have described the development of the first ER-targeted fluorescent H2S probe (>Na-H >2 >S-ER). The new probe exhibited favorable features, such as a large turn-on fluorescence signal (45-fold fluorescence enhancement), high sensitivity and selectivity. The probe was successfully employed for imaging exogenous and endogenous H2S in the living HeLa cells. Significantly, the new probe >Na-H >2 >S-ER was employed to visualize H2S in the ER of living cells for the first time. In addition, the probe was also successfully used for imaging H2S in the living tissues up to a depth of 100 μm and in the living zebrafish.
机译：硫化氢（H2S）作为重要的气体信号分子之一，与生物体内许多重要的生理过程有关。细胞器，尤其是内质网（ER），在细胞代谢中起关键作用。因此，在急诊室中检测H2S非常重要。为了实现这一目标，我们描述了第一个针对ER的荧光H2S探针（> Na-H > 2 > S-ER ）的开发。新探针具有良好的功能，例如大的开启荧光信号（增强了45倍的荧光），高灵敏度和选择性。该探针已成功用于成像活HeLa细胞中的外源性和内源性H2S。重要的是，首次使用新探针> Na-H > 2 > S-ER 可视化活细胞ER中的H2S。此外，该探针还成功用于在深度达100μm的活组织和斑马鱼中对H2S成像。

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