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首页> 美国卫生研究院文献>Scientific Reports >Effects of ultrasonication on increased germination and improved seedling growth of aged grass seeds of tall fescue and Russian wildrye
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Effects of ultrasonication on increased germination and improved seedling growth of aged grass seeds of tall fescue and Russian wildrye

机译：超声处理对高羊茅和俄罗斯野草衰老草种子发芽及幼苗生长的影响

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摘要

The effects of ultrasonic treatments on the germination and seedling growth of aged tall fescue (Festuca arundinacea) and Russian wild rye (Psathyrostaehys juncea Nevski) seeds were determined using orthogonal matrix experimental design with four ultrasonic factors. The multivariate analysis of variance detected significant differences and coupling effects of the pair-wise factors. The activities of Superoxide Dismutase (SOD) and Peroxidase (POD) and the Malondialdehyde (MDA) content were affected. The ultrasonic treatments had positive effects on the germination percentage (GP) of the aged seeds and the growth of the seedlings (GS) and therefore we provided a basic evidence for the application of ultrasonic treatment to pretreat aged grass seeds. For the four ultrasonic factors, the optimal conditions were a sonication time of 36.7 min, a sonication temperature of 35 °C, an output power of 367 W and a seed soaking time 4.1 h after binary quadratic regressions analyses. The ultrasonic treatment has the potential to improve seedling growth. Moreover, the longevity of the tall fescue and the Russian wild rye seeds was approximately 9.5 and 11.5 years, respectively, under natural conditions of storage. The physiological mechanisms that might contribute to the improved GP and GS were discussed.
机译：采用正交矩阵实验设计，结合四个超声因素，确定了超声处理对高羊茅（Festuca arundinacea）和俄罗斯野生黑麦（Psathyrostaehys juncea Nevski）种子发芽和幼苗生长的影响。方差的多变量分析检测到成对因素的显着差异和耦合效应。超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性以及丙二醛（MDA）的含量受到影响。超声波处理对衰老种子的发芽率（GP）和幼苗生长（GS）产生积极影响，因此，我们提供了超声波处理对老化草种子进行预处理的基础证据。对于这四个超声因素，最佳条件是经过二次二次回归分析后，超声处理时间为36.7 min，超声处理温度为35 C，输出功率为367 W，浸种时间为4.1 h。超声处理具有改善幼苗生长的潜力。此外，在自然储存条件下，高羊茅和俄罗斯野生黑麦种子的寿命分别约为9.5年和11.5年。讨论了可能有助于改善GP和GS的生理机制。

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