• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Scientific Reports >Methyl jasmonate induction of tanshinone biosynthesis in Salvia miltiorrhiza hairy roots is mediated by JASMONATE ZIM-DOMAIN repressor proteins
【2h】

Methyl jasmonate induction of tanshinone biosynthesis in Salvia miltiorrhiza hairy roots is mediated by JASMONATE ZIM-DOMAIN repressor proteins

机译：茉莉酸甲酯诱导丹参毛状根丹参酮生物合成的茉莉酸甲酯是由茉莉酸甲酯ZIM-DOMAIN阻遏蛋白介导的

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Jasmonic acid (JA) is an important plant hormone involved in regulation of many aspects of plant growth and development including secondary metabolism and JASMONATE ZIM-DOMAIN (JAZ) proteins are key components in JA signal processes. In this study, two new JAZ genes named SmJAZ3 and SmJAZ9 were cloned from S. miltiorrhiza hairy roots and characterized. Expression profiles under methyl jasmonate (MJ) treatment revealed that SmJAZ3 and SmJAZ9 were both MJ-responsive. Subcellular localization assay showed that SmJAZ3 was located in nucleus while SmJAZ9 was preferentially in nucleus. Expression of SmJAZ3 and SmJAZ9 in S. miltiorrhiza hairy roots differently affected the production of tanshinone. Over-expression of SmJAZ3 or SmJAZ9 in hairy roots produced lower level of tanshinone compared with the control, tanshinone production was as low as 0.077 mg/g DW in line SmJAZ3-3 and 0.266 mg/g DW in line SmJAZ9-22. Whereas, down-regulation of SmJAZs enhanced tanshione production, the content of tanshinone increased to 2.48 fold in anti-SmJAZ3-3 line, and 1.35-fold in anti-SmJAZ9-23 line. Our work indicated that SmJAZ3 and SmJAZ9 are involved in regulation of tanshinone biosynthesis and act as repressive transcriptional regulators in the JA signaling pathway, which paves the way to further dissect molecular mechanism in details in the future.
机译：茉莉酸（JA）是重要的植物激素，参与植物生长和发育的许多方面的调控，包括次级代谢，而JASMONATE ZIM-DOMAIN（JAZ）蛋白是JA信号过程中的关键成分。在这项研究中，从S. miltiorrhiza毛状根中克隆了两个新的JAZ基因，分别命名为SmJAZ3和SmJAZ9。茉莉酸甲酯（MJ）处理下的表达谱表明，SmJAZ3和SmJAZ9都是MJ反应的。亚细胞定位分析表明，SmJAZ3位于细胞核中，而SmJAZ9位于细胞核中。 SmJAZ3和SmJAZ9在丹参毛状根中的表达不同地影响丹参酮的产生。与对照相比，毛状根中SmJAZ3或SmJAZ9的过表达产生较低水平的丹参酮，SmJAZ3-3系丹参酮产量低至0.077μmg/ g DW，SmJAZ9-22系低至0.266μmg/ g DW。 SmJAZs的下调增强了丹参酮的产生，在抗 SmJAZ3-3 系中丹参酮的含量增加到2.48倍，在抗SmJAZ9-23 中丹参酮的含量增加1.35倍。 >行。我们的工作表明， SmJAZ3 和 SmJAZ9 参与丹参酮生物合成的调控，并在JA信号通路中起抑制性转录调节剂的作用，从而为进一步剖析其分子机制奠定了基础。未来的细节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号