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首页> 美国卫生研究院文献>Scientific Reports >Genome-wide analysis of the Dof gene family in durian reveals fruit ripening-associated and cultivar-dependent Dof transcription factors
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Genome-wide analysis of the Dof gene family in durian reveals fruit ripening-associated and cultivar-dependent Dof transcription factors

机译：对榴莲中Dof基因家族的全基因组分析揭示了果实成熟相关和品种依赖的Dof转录因子

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摘要

DNA binding with one finger (Dof) proteins constitute a ubiquitous plant-specific transcription factor (TF) family associated with diverse biological processes, including ripening. We conducted a genome-wide analysis of durian (Durio zibethinus Murr.) and identified 24 durian Dofs (DzDofs), 15 of which were expressed in fruit pulp. Gene expression analysis revealed differential expression of DzDofs during ripening in two commercial durian cultivars from Thailand, Monthong and Chanee. Comparing the expression levels of fruit pulp-expressed DzDofs between cultivars revealed ten potential cultivar-dependent Dofs, among which DzDof2.2 showed a significantly greater fold increase at every ripening stage in Chanee than in Monthong. The prediction of DzDof2.2’s function based on its orthologue in Arabidopsis revealed its possible role in regulating auxin biosynthesis. We observed significantly higher auxin levels during ripening of Chanee than Monthong which concurred with the greater expression of auxin biosynthetic genes. Transient expression of DzDof2.2 in Nicotiana benthamiana significantly upregulated the expression levels of auxin biosynthetic genes. Higher expression levels of DzDof2.2 in Chanee would enhance auxin levels through transcriptional regulation of auxin biosynthetic genes. Higher auxin levels in Chanee could activate auxin-mediated transcription, contributing to its faster ripening compared to Monthong through earlier initiation of the ethylene response (auxin-ethylene crosstalk).
机译：用一根手指（Dof）蛋白结合的DNA构成了一个普遍存在的植物特异性转录因子（TF）家族，与多种生物学过程相关，包括成熟。我们对榴莲（Durio zibethinus Murr。）进行了全基因组分析，确定了24个榴莲自由度（DzDofs），其中15个在果肉中表达。基因表达分析显示，来自泰国的两个商业榴莲品种Monthong和Chanee在成熟过程中DzDofs的差异表达。比较品种间果肉表达的DzDofs的表达水平，发现了十种潜在的依赖品种的Dofs，其中DzDof2.2在Chanee的每个成熟阶段的折叠倍数均显着高于Monthong。根据拟南芥中直向同源物对DzDof2.2功能的预测，揭示了其在调节植物生长素生物合成中的作用。我们观察到Chanee成熟期间的生长素水平明显高于Monthong，这与生长素生物合成基因的更高表达相一致。 DzDof2.2在本氏烟草中的瞬时表达显着上调了植物生长素生物合成基因的表达水平。 Chanee中较高的DzDof2.2表达水平将通过植物生长素生物合成基因的转录调控来提高植物生长素水平。 Chanee中较高的植物生长素水平可以激活植物生长素介导的转录，与Monthong相比，通过提前引发乙烯反应（植物生长素-乙烯串扰），使其成熟更快。

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