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首页> 美国卫生研究院文献>Scientific Reports >Common midwife toad ranaviruses replicate first in the oral cavity of smooth newts (Lissotriton vulgaris) and show distinct strain-associated pathogenicity
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Common midwife toad ranaviruses replicate first in the oral cavity of smooth newts (Lissotriton vulgaris) and show distinct strain-associated pathogenicity

机译：常见的助产士蟾蜍蛙病毒首先在平滑new（Lissotriton vulgaris）的口腔中复制并显示出与菌株相关的独特致病性

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Ranavirus is the second most common infectious cause of amphibian mortality. These viruses affect caudates, an order in which information regarding Ranavirus pathogenesis is scarce. In the Netherlands, two strains (CMTV-NL I and III) were suspected to possess distinct pathogenicity based on field data. To investigate susceptibility and disease progression in urodeles and determine differences in pathogenicity between strains, 45 adult smooth newts (Lissotriton vulgaris) were challenged via bath exposure with these ranaviruses and their detection in organs and feces followed over time by PCR, immunohistochemistry and in situ hybridization. Ranavirus was first detected at 3 days post infection (p.i.) in the oral cavity and upper respiratory mucosa. At 6 days p.i, virus was found in connective tissues and vasculature of the gastrointestinal tract. Finally, from 9 days p.i onwards there was widespread Ranavirus disease in various organs including skin, kidneys and gonads. Higher pathogenicity of the CMTV-NL I strain was confirmed by higher correlation coefficient of experimental group and mortality of challenged animals. Ranavirus-exposed smooth newts shed virus in feces intermittently and infection was seen in the absence of lesions or clinical signs, indicating that this species can harbor subclinical infections and potentially serve as disease reservoirs.
机译：鼻病毒是两栖动物死亡率的第二大常见传染原因。这些病毒会影响尾状病毒，而有关尾状病毒发病机理的信息却很少。在荷兰，根据现场数据，怀疑有两种菌株（CMTV-NL I和III）具有不同的致病性。为了研究尿de的易感性和疾病进展，并确定菌株之间的致病性差异，对45只成年平滑new（Lissotriton vulgaris）进行了暴雨病毒浸浴攻击，并通过PCR，免疫组化和原位杂交对器官和粪便进行了检测。感染后3天（p.i.）在口腔和上呼吸道粘膜中首次检测到了鼻病毒。下午6天，在胃肠道的结缔组织和脉管系统中发现了病毒。最后，从下午9天开始，在皮肤，肾脏和性腺等各个器官中都出现了广泛的Ranavirus疾病。实验组较高的相关系数和受攻击动物的死亡率证实了CMTV-NL I株的较高致病性。暴露于拉纳病毒的光滑new间歇地在粪便中散发病毒，并且在没有病灶或临床体征的情况下看到了感染，这表明该物种可以进行亚临床感染，并有可能成为疾病的宿主。

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