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首页> 美国卫生研究院文献>Scientific Reports >Purification of 3 4-dihydroxyphenylethyl alcohol glycoside from Sargentodoxa cuneata (Oliv.) Rehd. et Wils. and its protective effects against DSS-induced colitis
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Purification of 3 4-dihydroxyphenylethyl alcohol glycoside from Sargentodoxa cuneata (Oliv.) Rehd. et Wils. and its protective effects against DSS-induced colitis

机译：从Sargentodoxa cuneata（Oliv。）Rehd纯化34-二羟基苯乙醇糖苷。 et Wils。及其对DSS引起的结肠炎的保护作用

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Sargentodoxa cuneata is a tropical plant used in traditional Chinese medicine to treat intestinal inflammation. In this study, 3, 4-dihydroxyphenylethyl alcohol glycoside (DAG) was purified from the stem of S. cuneata using macroporous resins and its bioactivity was also investigated. The adsorption/desorption of DAG on macroporous resins was investigated systematically. HPD300 resin was selected as the most suitable medium for DAG purification. Further dynamic absorption/desorption experiments on the HPD300 column were conducted to obtain the optimal parameters. To obtain more than 95% DAG, a second stage procedure was developed to purify the DAG using SiliaSphere C18 with 8% v/v acetonitrile through elution at low pressure. Further investigation showed that DAG pretreatment significantly reversed the shortening of colon length, the increase in the disease activity index (DAI) scores and histological damage in the colon. Moreover, DAG greatly increased SOD and GPx activities, significantly decreased MPO and MDA activities and reduced the levels of pro-inflammatory cytokines in the colon. Free radical scavenging activities of DAG were assessed using DPPH, with an IC50 value of 17.03 ug/mL. Additionally, DAG suppressed ROS and proinflammatory cytokine production in LPS-stimulated RAW 264.7 macrophages by suppressing activation of the ERK1/2 and NF-κB pathways. The results were indicative of the antioxidant and anti-inflammatory properties of DAG. When viewed together, these findings indicated that DAG can be used to expand future pharmacological research and to potentially treat colitis.
机译：Sargentodoxa cuneata是一种热带植物，在传统中药中用于治疗肠道炎症。在这项研究中，使用大孔树脂从苦瓜的茎中纯化了3，4-二羟基苯乙醇糖苷（DAG），并对其生物活性进行了研究。系统地研究了DAG在大孔树脂上的吸附/解吸。选择HPD300树脂作为最适合DAG纯化的介质。在HPD300色谱柱上进行了进一步的动态吸收/解吸实验，以获得最佳参数。为了获得超过95％的DAG，开发了第二步程序以使用SiliaSphere C18和8％v / v乙腈通过低压洗脱纯化DAG。进一步的研究表明，DAG预处理可显着逆转结肠长度的缩短，疾病活动指数（DAI）分数的增加以及结肠的组织学损害。此外，DAG大大提高了SOD和GPx活性，大大降低了MPO和MDA活性，并降低了结肠中促炎性细胞因子的水平。使用DPPH评估DAG的自由基清除活性，IC50值为17.03 ug / mL。此外，DAG通过抑制ERK1 / 2和NF-κB途径的激活，抑制LPS刺激的RAW 264.7巨噬细胞中ROS和促炎性细胞因子的产生。结果表明DAG的抗氧化和抗炎特性。一起查看时，这些发现表明DAG可用于扩展未来的药理研究并潜在地治疗结肠炎。

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