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SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension

机译：SFTA3 –一种新型的眼表表面活性剂蛋白它在角膜伤口愈合和泪膜表面张力中的作用

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摘要

The study aimed to characterize the expression and function of SFTA3 at the ocular surface and in tears. Ocular tissues, conjunctival (HCjE) and human corneal (HCE) epithelial cell lines as well as tearfilm of patients suffering from different forms of dry eye disease (DED) were analyzed by means of RT-PCR, western blot, immunohistochemistry, and ELISA. A possible role of recombinant SFTA3 in corneal wound healing was investigated performing in vitro scratch assays. Tear film regulatory properties were analyzed with the spinning drop method and the regulation of SFTA3 transcripts was studied in HCE and HCjE after incubation with proinflammatory cytokines as well as typical ocular pathogens by real-time RT-PCR and ELISA. The results reveal that human ocular tissue as well as tears of healthy volunteers express SFTA3 whereas tears from patients with DED showed significantly increased SFTA3 levels. In vitro wounding of HCE cell cultures that had been treated with recombinant SFTA3 demonstrated a significantly increased wound closure rate and rSFTA3 reduced the surface tension of tear fluid. The results indicate that SFTA3 at the ocular surface seemed to be involved in wound healing and the reduction of surface tension.
机译：该研究旨在表征SFTA3在眼表和眼泪中的表达和功能。通过RT-PCR，Western印迹，免疫组织化学和ELISA分析了患有不同形式的干眼病（DED）的患者的眼组织，结膜（HCjE）和人角膜（HCE）上皮细胞系以及泪膜。进行体外划痕试验研究了重组SFTA3在角膜伤口愈合中的可能作用。通过纺丝滴落法分析了泪膜的调节特性，并通过实时RT-PCR和ELISA与促炎细胞因子以及典型的眼病菌孵育后，在HCE和HCjE中研究了SFTA3转录物的调节。结果表明，人眼组织以及健康志愿者的眼泪都表达SFTA3，而DED患者的眼泪表明SFTA3水平显着增加。用重组SFTA3处理的HCE细胞培养物的体外伤口显示伤口闭合率显着提高，而rSFTA3降低了泪液的表面张力。结果表明，眼表面的SFTA3似乎与伤口愈合和表面张力的降低有关。

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