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首页> 美国卫生研究院文献>Scientific Reports >Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium
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Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium

机译：IV型胶原α6链是口腔粘膜上皮角化过程中角蛋白10的调节剂

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Keratinized mucosa is of fundamental importance to maintain healthy gingival tissue, and understanding the mechanisms of oral mucosa keratinization is crucial to successfully manage healthy gingiva. Previous studies have shown a strong involvement of the basement membrane in the proliferation and differentiation of epithelial cells. Therefore, first, to identify the keratinized mucosa-specific basement membrane components, immunohistochemical analysis for the six alpha chains of type IV collagen was performed in 8-week-old mice. No difference in the expression pattern of type IV collagen α1(IV) and α2(IV) chains was observed in the keratinized and non-keratinized mucosa. Interestingly, however, type IV collagen α5(IV) and α6(IV) chains specifically were strongly detected in the keratinized mucosa. To analyze the functional roles of the type IV collagen isoform α6(IV) in oral mucosa keratinization, we analyzed Col4a6-knockout mice. Epithelial developmental delay and low levels of KRT10 were observed in new-born Col4a6-knockout mice. Additionally, in vitro experiments with loss-of function analysis using human gingival epithelial cells confirmed the important role of α6(IV) chain in epithelial keratinization. These findings indicate that α112:α556 (IV) network, which is the only network that includes the α6(IV) chain, is one regulator of KRT10 expression in keratinization of oral mucosal epithelium.
机译：角化黏膜对于维持健康的牙龈组织至关重要，而了解口腔黏膜角化的机制对于成功管理健康的牙龈至关重要。先前的研究表明，基底膜强烈参与上皮细胞的增殖和分化。因此，首先，为了鉴定角质化的粘膜特异性基底膜成分，在8周龄的小鼠中对IV型胶原的6条α链进行了免疫组织化学分析。在角化和未角化的粘膜中未观察到IV型胶原α1（IV）和α2（IV）链的表达方式的差异。然而，有趣的是，在角化粘膜中强烈检测到IV型胶原α5（IV）和α6（IV）链。为了分析IV型胶原蛋白同工型α6（IV）在口腔粘膜角化中的功能，我们分析了Col4a6-敲除小鼠。在新生的Col4a6基因敲除小鼠中观察到上皮发育延迟和KRT10水平低。此外，使用人牙龈上皮细胞进行功能丧失分析的体外实验证实了α6（IV）链在上皮角化中的重要作用。这些发现表明，α112：α556（IV）网络是唯一包含α6（IV）链的网络，是口腔粘膜上皮角化过程中KRT10表达的一种调节剂。

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