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SLAF-based high-density genetic map construction and QTL mapping for major economic traits in sea urchin Strongylocentrotus intermedius

机译:基于SLAF的高密度遗传图谱构建和海胆主要经济性状的QTL定位

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摘要

Sea urchin (Strongylocentrotus intermedius) has long been a model species for developmental and evolutionary research, but only a few studies have focused on gene mapping. Here, we reported a high-density genetic map containing 4,387 polymorphism specific-length amplified fragment (SLAF) markers spanning 21 linkage groups (LG) for sea urchin. Based on this genetic map and phenotyping data for eight economic traits, 33 potentially significant QTLs were detected on ten different LGs with explanations ranging from 9.90% to 46.30%, partly including 10 QTLs for test diameter, six QTLs for body weight and eight QTLs for Aristotle’s lantern weight. Moreover, we found a QTL enrichment LG, LG15, gathering QTLs for test diameter, body weight, gonad weight, light orange-yellow color difference (≥E1) and light yellow color difference (≥E2). Among all QTLs, we genotyped four QTLs for test diameter, Aristotle’s lantern weight and body weight using High Resolution Melting (HRM) technology. Finally, we used the verified SNP marker (detected using SLAF sequencing) to explore their marker-assisted selection (MAS) breeding application potential and found that SNP-29 associated tightly with body weight and that heterozygous genotype was a dominant genotype, indicating that SNP-29 was a promising marker for MAS.
机译:长期以来,海胆(Strongylocentrotus intermedius)一直是开发和进化研究的模型物种,但是只有少数研究集中在基因定位上。在这里,我们报告了一个高密度的遗传图谱,其中包含4,387个多态性特定长度扩增片段(SLAF)标记,这些标记跨越21个海胆连锁组(LG)。根据此遗传图谱和八个经济性状的表型数据,在十个不同的LG中检测到33个潜在的显着QTL,其解释范围从9.90%到46.30%,部分包括10个测试直径的QTL,6个体重的QTL和8个QTL。亚里斯多德的灯笼重量。此外,我们发现了一个QTL富集LG,LG15,收集了测试直径,体重,性腺重量,浅橙黄色色差(≥E1)和浅黄色色差(≥E2)的QTL。在所有QTL中,我们使用高分辨率熔解(HRM)技术对四个QTL进行了基因分型,分别用于测试直径,亚里斯多德的灯笼重量和体重。最后,我们使用经过验证的SNP标记(通过SLAF测序检测)来探索其标记辅助选择(MAS)的育种应用潜力,并发现SNP-29与体重紧密相关,并且杂合基因型是主要基因型,表明SNP -29是MAS的有前途的标志。

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