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首页> 美国卫生研究院文献>Scientific Reports >Enhancing size based size separation through vertical focus microfluidics using secondary flow in a ridged microchannel
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Enhancing size based size separation through vertical focus microfluidics using secondary flow in a ridged microchannel

机译：通过使用脊状微通道中的二次流通过垂直聚焦微流体增强基于尺寸的尺寸分离

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摘要

High throughput size based separation and sorting of bioparticles and cells is critical to a variety of biomedical processing steps for medical diagnostics and pharmaceutical purification. Improving microfluidic size-based particle/cell sorting is a challenge to better address the need for generating more homogeneous subpopulations for study and use. We propose a novel advance to microfluidic sorting devices that uses three-dimensional focusing of the sample to optimally position particles to amplify the size-dependent differences in trajectories caused by differential secondary flows. The result is an increase in the purity of small particles by 35- fold and large particles by 8-fold in comparison to unfocused flow. Our simulated and experimental data reveal for the first time that positioning particles in three-dimensional space can be used to better leverage the differential lateral movement of particles with different sizes as they flow in microchannel with transverse secondary flows. The focusing approach may also be useful to improve positioning of particles with inertial channels with multiple equilibrium positions. This technique performs continuous-flow, high throughput size based sorting of millions of particles and cells in a minute without any pre and post-processing. We have also demonstrated improved enrichment and recovery of white blood cells from human blood.
机译：基于高通量大小的生物颗粒和细胞的分离和分类对于医疗诊断和药物纯化的各种生物医学处理步骤至关重要。改善基于微流体尺寸的颗粒/细胞分选对更好地解决产生更均质的亚群用于研究和使用的需求是一个挑战。我们提出了一种微流体分选设备的新进展，该设备使用了样品的三维聚焦来最佳地定位粒子，以放大由差分二次流引起的轨迹中尺寸相关的差异。与未聚焦的流动相比，结果是小颗粒的纯度提高了35倍，大颗粒的纯度提高了8倍。我们的模拟和实验数据首次揭示了在三维空间中定位粒子可以更好地利用不同大小的粒子在横向横向二次流动中在微通道中流动时所产生的差分横向运动。聚焦方法还可用于改善具有具有多个平衡位置的惯性通道的粒子的定位。该技术可在一分钟内对数百万个粒子和细胞进行连续流，高通量大小的分选，而无需任何预处理和后处理。我们还证明了人血中白细胞的富集和回收得到改善。

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