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首页> 美国卫生研究院文献>Scientific Reports >Inhibiting P. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study
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Inhibiting P. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study

机译：含氟聚合物嵌入的银纳米粒子抑制荧光假单胞菌生物膜的原位光谱研究

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Surface colonization by microorganisms leads to the formation of biofilms, i.e. aggregates of bacteria embedded within a matrix of extracellular polymeric substance. This promotes adhesion to the surface and protects bacterial community, providing an antimicrobial-resistant environment. The inhibition of biofilm growth is a crucial issue for preventing bacterial infections. Inorganic nanoparticle/Teflon-like (CFx) composites deposited via ion beam sputtering demonstrated very efficient antimicrobial activity. In this study, we developed Ag-CFx thin films with tuneable metal loadings and exceptional in-plane morphological and chemical homogeneity. Ag-CFx antimicrobial activity was studied via mid-infrared attenuated total reflection spectroscopy utilizing specifically adapted multi-reflection waveguides. Biofilm was sampled by carefully depositing the Ag-CFx film on IR inactive regions of the waveguide. Real-time infrared spectroscopy was used to monitor Pseudomonas fluorescens biofilm growth inhibition induced by the bioactive silver ions released from the nanoantimicrobial coating. Few hours of Ag-CFx action were sufficient to affect significantly biofilm growth. These findings were corroborated by atomic force microscopy (AFM) studies on living bacteria exposed to the same nanoantimicrobial. Morphological analyses showed a severe bacterial stress, leading to membrane leakage/collapse or to extended cell lysis as a function of incubation time.
机译：微生物的表面定殖导致生物膜的形成，即嵌入细胞外聚合物基质中的细菌聚集体。这促进了对表面的粘附并保护了细菌群落，提供了抗微生物的环境。生物膜生长的抑制是防止细菌感染的关键问题。通过离子束溅射沉积的无机纳米粒子/类聚四氟乙烯（CFx）复合材料表现出非常有效的抗菌活性。在这项研究中，我们开发了具有可调节金属负载以及出色的面内形态和化学均匀性的Ag-CFx薄膜。 Ag-CFx的抗菌活性是通过中红外衰减全反射光谱法研究的，该光谱使用了专门设计的多反射波导。通过小心地将Ag-CFx膜沉积在波导的IR非活性区域上来采样生物膜。实时红外光谱用于监测由纳米抗菌涂层释放的生物活性银离子诱导的荧光假单胞菌生物膜生长抑制。几小时的Ag-CFx作用足以显着影响生物膜的生长。原子力显微镜（AFM）对暴露于同一纳米抗菌剂的活细菌的研究证实了这些发现。形态学分析显示，严重的细菌压力会导致膜渗漏/塌陷或细胞裂解时间延长，这是孵育时间的函数。

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