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首页> 美国卫生研究院文献>Scientific Reports >BioFlow: a non-invasive image-based method to measure speed pressure and forces inside living cells
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BioFlow: a non-invasive image-based method to measure speed pressure and forces inside living cells

机译：BioFlow：一种基于图像的非侵入性方法可测量活细胞内部的速度压力和作用力

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摘要

Cell motility is governed by a complex molecular machinery that converts physico-chemical cues into whole-cell movement. Understanding the underlying biophysical mechanisms requires the ability to measure physical quantities inside the cell in a simple, reproducible and preferably non-invasive manner. To this end, we developed BioFlow, a computational mechano-imaging method and associated software able to extract intracellular measurements including pressure, forces and velocity everywhere inside freely moving cells in two and three dimensions with high spatial resolution in a non-invasive manner. This is achieved by extracting the motion of intracellular material observed using fluorescence microscopy, while simultaneously inferring the parameters of a given theoretical model of the cell interior. We illustrate the power of BioFlow in the context of amoeboid cell migration, by modelling the intracellular actin bulk flow of the parasite Entamoeba histolytica using fluid dynamics, and report unique experimental measures that complement and extend both theoretical estimations and invasive experimental measures. Thanks to its flexibility, BioFlow is easily adaptable to other theoretical models of the cell, and alleviates the need for complex or invasive experimental conditions, thus constituting a powerful tool-kit for mechano-biology studies. BioFlow is open-source and freely available via the Icy software.
机译：细胞运动受复杂的分子机制控制，该机制将物理化学线索转换为全细胞运动。了解潜在的生物物理机制要求以简单，可复制且最好是非侵入性方式测量细胞内部物理量的能力。为此，我们开发了BioFlow，这是一种机械力成像计算方法，其相关的软件能够以无创方式在二维和三维空间内自由移动的细胞内，提取细胞内的测量值，包括压力，力和速度。这是通过提取使用荧光显微镜观察到的细胞内材料的运动来实现的，同时推断出细胞内部给定理论模型的参数。我们通过使用流体动力学建模寄生虫组织解组织变形杆菌的细胞内肌动蛋白大量流动，说明了在类氨醇细胞迁移的背景下BioFlow的功能，并报告了补充和扩展理论估计和侵入性实验措施的独特实验方法。由于BioFlow的灵活性，它很容易适应细胞的其他理论模型，并减轻了对复杂或侵入性实验条件的需求，因此构成了用于机械生物学研究的强大工具包。 BioFlow是开源的，可通过Icy软件免费获得。

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