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首页> 美国卫生研究院文献>Scientific Reports >Labeling and in vivo visualization of transplanted adipose tissue-derived stem cells with safe cadmium-free aqueous ZnS coating of ZnS-AgInS2 nanoparticles
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Labeling and in vivo visualization of transplanted adipose tissue-derived stem cells with safe cadmium-free aqueous ZnS coating of ZnS-AgInS2 nanoparticles

机译：ZnS-AgInS2纳米颗粒的安全无镉水性ZnS涂层对已移植脂肪组织干细胞的标记和体内可视化

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The facile synthesis of ZnS-AgInS2 (ZAIS) as cadmium-free QDs and their application, mainly in solar cells, has been reported by our groups. In the present study, we investigated the safety and the usefulness for labeling and in vivo imaging of a newly synthesized aqueous ZnS-coated ZAIS (ZnS-ZAIS) carboxylated nanoparticles (ZZC) to stem cells. ZZC shows the strong fluorescence in aqueous solutions such as PBS and cell culture medium, and a complex of ZZC and octa-arginine (R8) peptides (R8-ZZC) can achieve the highly efficient labeling of adipose tissue-derived stem cells (ASCs). The cytotoxicity of R8-ZZC to ASCs was found to be extremely low in comparison to that of CdSe-based QDs, and R8-ZZC was confirmed to have no influence on the proliferation rate or the differentiation ability of ASCs. Moreover, R8-ZZC was not found to induce the production of major inflammatory cytokines (TNF-α, IFN-γ, IL-12p70, IL-6 and MCP-1) in ASCs. Transplanted R8-ZZC-labeled ASCs could be quantitatively detected in the lungs and liver mainly using an in vivo imaging system. In addition, high-speed multiphoton confocal laser microscopy revealed the presence of aggregates of transplanted ASCs at many sites in the lungs, whereas individual ASCs were found to have accumulated in the liver.
机译：我们的研究小组已经报道了ZnS-AgInS2（ZAIS）作为无镉量子点的简便合成及其主要在太阳能电池中的应用。在本研究中，我们调查了新合成的水性ZnS涂层ZAIS（ZnS-ZAIS）羧化纳米颗粒（ZZC）对干细胞的标记和体内成像的安全性和实用性。 ZZC在PBS和细胞培养基等水溶液中显示强荧光，并且ZZC和八精氨酸（R8）肽（R8-ZZC）的复合物可以实现脂肪组织衍生干细胞（ASC）的高效标记。与基于CdSe的量子点相比，发现R8-ZZC对ASC的细胞毒性极低，并且证实R8-ZZC对ASC的增殖速率或分化能力没有影响。而且，未发现R8-ZZC在ASC中诱导主要炎症细胞因子（TNF-α，IFN-γ，IL-12p70，IL-6和MCP-1）的产生。可以使用体内成像系统在肺和肝中定量检测移植的R8-ZZC标记的ASC。此外，高速多光子共聚焦激光显微镜检查显示，肺中许多部位都存在移植的ASC聚集体，而单个ASC被发现在肝脏中积累。

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