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首页> 美国卫生研究院文献>Scientific Reports >Bis-three-way junction nanostructure and DNA machineries for ultrasensitive and specific detection of BCR/ABL fusion gene by chemiluminescence imaging
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Bis-three-way junction nanostructure and DNA machineries for ultrasensitive and specific detection of BCR/ABL fusion gene by chemiluminescence imaging

机译：化学发光成像技术可用于BCR / ABL融合基因的超灵敏和特异性检测的双向三向连接纳米结构和DNA机器

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摘要

A novel G-quadruplex DNAzyme-driven chemiluminescence (CL) imaging method has been developed for ultrasensitive and specific detection of BCR/ABL fusion gene based on bis-three-way junction (bis-3WJ) nanostructure and cascade DNA machineries. Bis-3WJ probes are designed logically to recognize BCR/ABL fusion gene, which forms the stable bis-3WJ nanostructure for the activation of polymeraseicking enzyme machineries in cascade, resulting in synthesis of DNAzyme subunits. These DNAzyme subunits can form integrated DNAzyme by self-assembly to catalyze CL substrate, thus providing an amplified signal for the sensing events or outputs for AND logic operation. The imaging method achieved ultrasensitive detection of BCR/ABL fusion gene with a low detection limit down to 23 fM. And this method exhibited wide linear ranges over seven orders of magnitude and excellent discrimination ability toward target. In addition, an acceptable recovery was obtained in complex matrix. It is notable that this biosensing strategy possesses merits of homogenous, isothermal and label-free assay system. Therefore, these merits endow the developed imaging method with a potential tool for CML diagnosis.
机译：已经开发了一种新颖的G-四链体DNA酶驱动的化学发光（CL）成像方法，用于基于双向三向连接（bis-3WJ）纳米结构和级联DNA机器的BCR / ABL融合基因的超灵敏和特异性检测。 Bis-3WJ探针在逻辑上设计为识别BCR / ABL融合基因，该基因形成稳定的bis-3WJ纳米结构，用于级联激活聚合酶/切口酶机械，从而合成DNAzyme亚基。这些DNAzyme亚基可通过自组装形成整合的DNAzyme，以催化CL底物，从而为检测事件或AND逻辑操作的输出提供放大的信号。该成像方法实现了BCR / ABL融合基因的超灵敏检测，检测限低至23 fM。并且该方法表现出超过七个数量级的宽线性范围，并且对目标具有出色的辨别能力。另外，在复杂基质中获得了可接受的回收率。值得注意的是，这种生物传感策略具有均质，等温且无标记的检测系统的优点。因此，这些优点使开发的成像方法具有用于CML诊断的潜在工具。

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