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首页> 美国卫生研究院文献>Scientific Reports >Adaptation of the autotrophic acetogen Sporomusa ovata to methanol accelerates the conversion of CO2 to organic products
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Adaptation of the autotrophic acetogen Sporomusa ovata to methanol accelerates the conversion of CO2 to organic products

机译：自养卵原菌卵形孢子虫（Sporomusa ovata）对甲醇的适应加速了CO2向有机产物的转化

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Acetogens are efficient microbial catalysts for bioprocesses converting C1 compounds into organic products. Here, an adaptive laboratory evolution approach was implemented to adapt Sporomusa ovata for faster autotrophic metabolism and CO2 conversion to organic chemicals. S. ovata was first adapted to grow quicker autotrophically with methanol, a toxic C1 compound, as the sole substrate. Better growth on different concentrations of methanol and with H2-CO2 indicated the adapted strain had a more efficient autotrophic metabolism and a higher tolerance to solvent. The growth rate on methanol was increased 5-fold. Furthermore, acetate production rate from CO2 with an electrode serving as the electron donor was increased 6.5-fold confirming that the acceleration of the autotrophic metabolism of the adapted strain is independent of the electron donor provided. Whole-genome sequencing, transcriptomic, and biochemical studies revealed that the molecular mechanisms responsible for the novel characteristics of the adapted strain were associated with the methanol oxidation pathway and the Wood-Ljungdahl pathway of acetogens along with biosynthetic pathways, cell wall components, and protein chaperones. The results demonstrate that an efficient strategy to increase rates of CO2 conversion in bioprocesses like microbial electrosynthesis is to evolve the microbial catalyst by adaptive laboratory evolution to optimize its autotrophic metabolism.
机译：乙酸原素是用于将C1化合物转化为有机产品的生物过程的有效微生物催化剂。在这里，实施了一种适应性实验室进化方法，以使卵形孢子菌（Sporomusa ovata）适应更快的自养代谢和将CO2转化为有机化学品。卵形链球菌首先适合以甲醇（一种有毒的C1化合物）为唯一底物，更快地自养。在不同浓度的甲醇上和使用H2-CO2的情况下，更好的生长表明适应的菌株具有更有效的自养代谢和对溶剂的更高耐受性。在甲醇上的生长速率增加了5倍。此外，使用电极作为电子给体的CO2乙酸盐产生速率提高了6.5倍，这证实了适应菌株自养代谢的加速与提供的电子给体无关。全基因组测序，转录组学和生化研究表明，负责适应菌株新特性的分子机制与乙酸原的甲醇氧化途径和Wood-Ljungdahl途径以及生物合成途径，细胞壁成分和蛋白质有关伴侣。结果表明，在诸如微生物电合成的生物过程中提高CO2转化率的有效策略是通过适应性实验室进化来发展微生物催化剂，以优化其自养代谢。

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