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首页> 美国卫生研究院文献>Scientific Reports >Effect of carbonic anhydrase on silicate weathering and carbonate formation at present day CO2 concentrations compared to primordial values
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Effect of carbonic anhydrase on silicate weathering and carbonate formation at present day CO2 concentrations compared to primordial values

机译：与原始值相比碳酸酐酶对当今CO2浓度下硅酸盐风化和碳酸盐形成的影响

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摘要

It is widely recognized that carbonic anhydrase (CA) participates in silicate weathering and carbonate formation. Nevertheless, it is still not known if the magnitude of the effect produced by CA on surface rock evolution changes or not. In this work, CA gene expression from Bacillus mucilaginosus and the effects of recombination protein on wollastonite dissolution and carbonate formation under different conditions are explored. Real-time fluorescent quantitative PCR was used to explore the correlation between CA gene expression and sufficiency or deficiency in calcium and CO2 concentration. The results show that the expression of CA genes is negatively correlated with both CO2 concentration and ease of obtaining soluble calcium. A pure form of the protein of interest (CA) is obtained by cloning, heterologous expression, and purification. The results from tests of the recombination protein on wollastonite dissolution and carbonate formation at different levels of CO2 concentration show that the magnitudes of the effects of CA and CO2 concentration are negatively correlated. These results suggest that the effects of microbial CA in relation to silicate weathering and carbonate formation may have increased importance at the modern atmospheric CO2 concentration compared to 3 billion years ago.
机译：众所周知，碳酸酐酶（CA）参与了硅酸盐的风化作用和碳酸盐的形成。然而，仍然不知道CA对地表岩石演化的影响大小是否改变。在这项工作中，探索了黏液芽孢杆菌的CA基因表达以及重组蛋白在不同条件下对硅灰石溶解和碳酸盐形成的影响。实时荧光定量PCR用于探讨CA基因表达与钙和CO2浓度是否充足之间的相关性。结果表明，CA基因的表达与CO 2浓度和获得可溶性钙的难易程度均呈负相关。通过克隆，异源表达和纯化获得目标蛋白（CA）的纯形式。重组蛋白在不同浓度的CO2下对硅灰石溶解和碳酸盐形成的测试结果表明，CA和CO2浓度的影响幅度呈负相关。这些结果表明，与30亿年前相比，在现代大气CO2浓度下，微生物CA对硅酸盐风化和碳酸盐形成的影响可能越来越重要。

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