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High Prevalence of Panton-Valentine Leukocidin Among Methicillin-Sensitive Staphylococcus aureus Colonization Isolates in Rural Iowa

机译:在爱荷华州农村地区对甲氧西林敏感的金黄色葡萄球菌定植菌株中潘通-华伦丁白蛋白的患病率很高

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摘要

Recent studies have shown that livestock can carry Staphylococcus aureus and transmit it to human caretakers. We conducted a pilot study to determine the prevalence and molecular epidemiology of S. aureus among rural Iowans, including individuals with livestock contact. Nasal and throat swabs were collected and plated onto selective media to isolate methicillin-susceptible and methicillin-resistant S. aureus (MRSA), followed by antibiotic resistance testing and molecular analysis of the isolates. While no MRSA was detected, overall, 23.7% (31/131) of participants were found to harbor S. aureus in their nose, throat, or both. Fifteen isolates displayed resistance to one or more tested antibiotics, and the Panton-Valentine leukocidin (PVL) genes were present at a high level (29% [9/31] of S. aureus-positive participants). Younger age and tobacco use were associated with increased risk of S. aureus carriage. Our results suggest that carriage of PVL-positive S. aureus is common among rural Iowans, even in the absence of detectable MRSA colonization.
机译:最近的研究表明,牲畜可以携带金黄色葡萄球菌并将其传播给人类看护人。我们进行了一项初步研究,以确定在爱荷华州农村地区,包括与牲畜接触的个体中金黄色葡萄球菌的流行和分子流行病学。收集鼻和咽拭子,并将其铺在选择性培养基上,以分离出对甲氧西林敏感和耐甲氧西林的金黄色葡萄球菌(MRSA),然后进行抗生素抗性测试和分离物的分子分析。虽然未检测到MRSA,但总的来说,发现23.7%(31/131)的参与者的鼻子,喉咙或两者中都带有金黄色葡萄球菌。 15个分离株对一种或多种测试抗生素表现出抗药性,并且Panton-Valentine leukocidin(PVL)基因的含量很高(金黄色葡萄球菌阳性参与者中29%[9/31])。年龄较小和吸烟与金黄色葡萄球菌携带风险增加有关。我们的结果表明,PVL阳性金黄色葡萄球菌的运输在爱荷华州农村地区很普遍,即使没有可检测的MRSA定植。

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