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首页> 美国卫生研究院文献>Journal of Neurophysiology >Control of Movement: Muscle afferent excitability testing in spinal root-intact rats: dissociating peripheral afferent and efferent volleys generated by intraspinal microstimulation
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Control of Movement: Muscle afferent excitability testing in spinal root-intact rats: dissociating peripheral afferent and efferent volleys generated by intraspinal microstimulation

机译：运动控制：脊髓根完整的大鼠的肌肉传入兴奋性测试：分离脊髓内微刺激产生的外周传入和传出凌空

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Presynaptic inhibition of the sensory input from the periphery to the spinal cord can be evaluated directly by intra-axonal recording of primary afferent depolarization (PAD) or indirectly by intraspinal microstimulation (excitability testing). Excitability testing is superior for use in normal behaving animals, because this methodology bypasses the technically challenging intra-axonal recording. However, use of excitability testing on the muscle or joint afferent in intact animals presents its own technical challenges. Because these afferents, in many cases, are mixed with motor axons in the peripheral nervous system, it is crucial to dissociate antidromic volleys in the primary afferents from orthodromic volleys in the motor axon, both of which are evoked by intraspinal microstimulation. We have demonstrated in rats that application of a paired stimulation protocol with a short interstimulus interval (ISI) successfully dissociated the antidromic volley in the nerve innervating the medial gastrocnemius muscle. By using a 2-ms ISI, the amplitude of the volleys evoked by the second stimulation was decreased in dorsal root-sectioned rats, but the amplitude did not change or was slightly increased in ventral root-sectioned rats. Excitability testing in rats with intact spinal roots indicated that the putative antidromic volleys exhibited dominant primary afferent depolarization, which was reasonably induced from the more dorsal side of the spinal cord. We concluded that excitability testing with a paired-pulse protocol can be used for studying presynaptic inhibition of somatosensory afferents in animals with intact spinal roots.>NEW & NOTEWORTHY Excitability testing of primary afferents has been used to evaluate presynaptic modulation of synaptic transmission in experiments conducted in vivo. However, to apply this method to muscle afferents of animals with intact spinal roots, it is crucial to dissociate antidromic and orthodromic volleys induced by spinal microstimulation. We propose a new method to make this dissociation possible without cutting spinal roots and demonstrate that it facilitates excitability testing of muscle afferents.
机译：突触前对从外周到脊髓的感觉输入的抑制可通过原发传入去极化（PAD）的轴突内记录直接评估，或通过椎间微刺激（兴奋性测试）间接评估。兴奋性测试在正常行为动物中的使用是优越的，因为这种方法绕过了技术上具有挑战性的轴突内记录。但是，对完整动物的肌肉或传入关节进行兴奋性测试存在其自身的技术挑战。因为在许多情况下，这些传入神经与周围神经系统中的运动轴突混合在一起，所以至关重要的是，将初次传入的抗线性截齿与运动轴突中的正畸性截齿分开，这两者都是通过椎间微刺激诱发的。我们已经在大鼠中证明，配对刺激方案与短刺激间隔（ISI）的应用成功地使神经支配性凌空性神经支配了腓肠肌内侧神经。通过使用2毫秒ISI，在背根切开的大鼠中第二次刺激引起的凌空幅度降低，但在腹根切开的大鼠中该幅度不变或略有增加。在具有完整脊髓根的大鼠中进行的兴奋性测试表明，推定的反运动截击表现出显性的初级传入去极化，这是从脊髓较背面侧合理诱导的。我们得出的结论是，使用成对脉冲协议进行兴奋性测试可用于研究具有完整脊椎根的动物的突触前抑制。> NEW＆NOTEWORTHY 主要的兴奋性兴奋性测试已用于评估突触前调节。在体内进行的实验中突触传递的变化。但是，要将这种方法应用于具有完整脊柱根的动物的肌肉传入，分离由脊柱微刺激诱导的反峰和正峰的截击至关重要。我们提出了一种新方法，使这种分离成为可能而无需切断脊椎根，并证明它有助于肌肉传入神经的兴奋性测试。

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