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High‐resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile

机译：粪便DNA中细胞色素B基因的高分辨率融合：智利Lycalopex属狐狸种鉴定的有力方法

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摘要

Easy, economic, precise species authentication is currently necessary in many areas of research and diagnosis in molecular biology applied to conservation studies of endangered species. Here, we present a new method for the identification of three fox species of the Lycalopex genus in Chile. We developed an assay based on high‐resolution melt analysis of the mitochondrial cytochrome B gene, allowing a simple, low cost, fast, and accurate species determination. To validate the assay applicability for noninvasive samples, we collected fecal samples in the Atacama Desert, finding unexpectedly one species outside of its known distribution range. We conclude that the assay has a potential to become a valuable tool for a standardized genetic monitoring of the Lycalopex species in Chile.

机译：目前，在分子生物学的许多研究和诊断领域中，需要简单，经济，精确地进行物种鉴定，以应用于濒危物种的保护研究。在这里，我们提出了一种新的方法来鉴定智利Lycalopex属的三种狐狸种。我们基于线粒体细胞色素B基因的高分辨率熔解分析开发了一种测定方法，可进行简单，低成本，快速且准确的物种确定。为了验证该分析方法对非侵入性样品的适用性，我们在阿塔卡马沙漠中收集了粪便样品，出乎意料地发现了一种不在其已知分布范围内的物种。我们得出的结论是，该分析方法有可能成为标准化智利Lycalopex物种遗传监测的有价值的工具。

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期刊名称 Ecology and Evolution
作者
Leonardo Anabalón; Francisco Encina‐Montoya; Pamela Sánchez; Jaime Solano; Felipe Benavente; Basilio Guiñez; Flavio Olivares; Carlos Oberti; Rolando Vega;
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作者单位

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年(卷),期 2019(9),13
年度 2019
页码 7448–7454
总页数 7
原文格式 PDF
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中图分类
关键词
barcode HRM Lycalopex PCR;

机译：条码;HRM;Lycalopex;PCR;

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专利

1. High‐resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile [J] . Leonardo Anabalón, Francisco Encina‐Montoya, Pamela Sánchez, Ecology and Evolution . 2019,第13期

机译：粪便DNA中细胞色素B基因的高分辨率融合：智利Lycalopex属狐狸种鉴定的有力方法
2. High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors [J] . Victor H. Pe?a, Geysson J. Fernández, Andrés M. Gómez-Palacio, PLOS Neglected Tropical Diseases . 2012,第2期

机译：细胞色素B基因的高分辨率熔解（HRM）：查加查加斯病载体中血粉来源的有力方法
3. A DNA-based approach to the identification of insect species used for postmortem interval estimation and partial sequencing of the cytochrome oxydase b subunit gene I: a tool for the identification of European species of blow flies for postmortem int [J] . Wells JD, Sperling FA Journal of forensic sciences. . 2000,第6期

机译：一种基于DNA的方法来鉴定用于死后间隔估计和细胞色素氧化酶b亚基基因I部分测序的昆虫物种：用于鉴定欧洲死后蝇of种的工具
4. Morphological and Molecular Identification of Sri Lankan Leishmania Species Based on Kinetoplast Minicircle DNA and Cytochrome b Gene from Human Isolates [C] . L. Yatawara, S. Wickramasinghe, R.P.V.J. Rajapakse, International Congress of Parasitology . 2006

机译：基于Kinetoplast Minicircle DNA和来自人分离株的细胞色素B基因的Sri Lankan Leishmania物种的形态学和分子鉴定
5. Identification and phylogenetic relationships within the genus Tetrahymena using the cytochrome c oxidase subunit 1 gene and the small subunit ribosomal RNA gene. [D] . Chantangsi, Chitchai. 2006

机译：使用细胞色素c氧化酶亚基1基因和小亚基核糖体RNA基因鉴定和鉴定四膜虫内的系统发育关系。
6. High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors [O] . Victor H. Peña, Geysson J. Fernández, Andrés M. Gómez-Palacio, 2012

机译：细胞色素B基因的高分辨率熔解（HRM）：查加查加斯病载体中血粉来源的有力方法
7. High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors [O] . Peña, Victor H., Fernández, Geysson J., Gómez-Palacio, Andrés M., 2012

机译：细胞色素B基因的高分辨率熔解（HRM）：查加查加斯病载体中血粉来源的有力方法

High‐resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile

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