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首页> 美国卫生研究院文献>Ecology and Evolution >A putative antimicrobial peptide from Hymenoptera in the megaplasmid pSCL4 of Streptomyces clavuligerus ATCC 27064 reveals a singular case of horizontal gene transfer with potential applications
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A putative antimicrobial peptide from Hymenoptera in the megaplasmid pSCL4 of Streptomyces clavuligerus ATCC 27064 reveals a singular case of horizontal gene transfer with potential applications

机译：棒状链霉菌ATCC 27064的大质粒pSCL4中膜翅目的推定抗菌肽揭示了水平基因转移的一种特殊情况具有潜在的应用前景

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Streptomyces clavuligerus is a Gram‐positive bacterium that is a high producer of secondary metabolites with industrial applications. The production of antibiotics such as clavulanic acid or cephamycin has been extensively studied in this species; nevertheless, other aspects, such as evolution or ecology, have received less attention. Furthermore, genes that arise from ancient events of lateral transfer have been demonstrated to be implicated in important functions of host species. This approximation discovered relevant genes that genomic analyses overlooked. Thus, we studied the impact of horizontal gene transfer in the S. clavuligerus genome. To perform this task, we applied whole‐genome analysis to identify a laterally transferred sequence from different domains. The most relevant result was a putative antimicrobial peptide (AMP) with a clear origin in the Hymenoptera order of insects. Next, we determined that two copies of these genes were present in the megaplasmid pSCL4 but absent in the S. clavuligerus ATCC 27064 chromosome. Additionally, we found that these sequences were exclusive to the ATCC 27064 strain (and so were not present in any other bacteria) and we also verified the expression of the genes using RNAseq data. Next, we used several AMP predictors to validate the original annotation extracted from Hymenoptera sequences and explored the possibility that these proteins had post‐translational modifications using peptidase cleavage prediction. We suggest that Hymenoptera AMP‐like proteins of S. clavuligerus ATCC 27064 may be useful for both species adaptation and as an antimicrobial molecule with industrial applications.
机译：克拉维链霉菌是革兰氏阳性细菌，在工业应用中是次生代谢产物的大量生产者。在该物种中已经广泛研究了抗生素的生产，例如棒酸或头霉素。但是，诸如进化或生态学等其他方面的关注较少。此外，已证明源自古代横向转移事件的基因与宿主物种的重要功能有关。这种近似发现了基因组分析忽略的相关基因。因此，我们研究了锁骨链霉菌基因组中水平基因转移的影响。为了执行此任务，我们应用了全基因组分析来识别来自不同域的横向转移序列。最相关的结果是推定的抗菌肽（AMP），其明显起源于昆虫的膜翅目。接下来，我们确定这些基因的两个副本存在于大质粒pSCL4中，但不存在于锁骨链球菌ATCC 27064染色体中。此外，我们发现这些序列是ATCC 27064菌株独有的（因此在其他细菌中不存在），并且我们还使用RNAseq数据验证了基因的表达。接下来，我们使用了几种AMP预测因子来验证从膜翅目序列中提取的原始注释，并使用肽酶裂解预测方法探讨了这些蛋白质具有翻译后修饰的可能性。我们建议S.clavuligerus ATCC 27064的膜翅目AMP样蛋白可能既可用于物种适应，又可作为工业应用中的抗菌分子。

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