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首页> 美国卫生研究院文献>Tissue Engineering and Regenerative Medicine >Different Bone Healing Effects of Undifferentiated and Osteogenic Differentiated Mesenchymal Stromal Cell Sheets in Canine Radial Fracture Model
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Different Bone Healing Effects of Undifferentiated and Osteogenic Differentiated Mesenchymal Stromal Cell Sheets in Canine Radial Fracture Model

机译：犬Rad骨骨折模型中未分化和成骨的间质基质细胞片的不同骨愈合效果

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Cell sheets technology is being available for fracture healing. This study was performed to clarify bone healing mechanism of undifferentiated (UCS) and osteogenic (OCS) differentiated mesenchymal stromal cell (MSC) sheets in the fracture model of dogs. UCS and OCS were harvested at 10 days of culture. Transverse fractures at the radius of six beagle dogs were assigned into three groups (n = 4 in each group) i.e. UCS, OCS and control. The fractures were fixed with a 2.7 mm locking plate and six screws. Cell sheets were wrapped around the fracture site. Bones were harvested 8 weeks after operation, then scanned by micro-computed tomography (micro-CT) and analyzed histopathologically. The micro-CT revealed different aspects of bone regeneration among the groups. The percentages of external callus volume out of total bone volume in control, UCS, and OCS groups were 42.1, 13.0 and 4.9% (p < 0.05) respectively. However, the percentages of limbs having connectivity of gaps were 25, 12.5 and 75% respectively. In histopathological assessments, OCS group showed well organized and mature woven bone with peripheral cartilage at the fracture site, whereas control group showed cartilage formation without bone maturation or ossification at the fracture site. Meanwhile, fracture site was only filled with fibrous connective tissue without endochondral ossification and bone formation in UCS group. It was suggested that the MSC sheets reduced the quantity of external callus, and OCS induced the primary bone healing.
机译：细胞片技术可用于骨折愈合。进行这项研究以阐明犬骨折模型中未分化（UCS）和成骨（OCS）分化的间充质基质细胞（MSC）片的骨愈合机制。培养10天时收获UCS和OCS。将六只比格犬半径的横向骨折分为三组（每组n = 4），即UCS，OCS和对照组。用2.7毫米锁定板和六个螺钉固定骨折。细胞片包裹在骨折部位周围。手术后8周收获骨骼，然后通过微型计算机断层扫描（micro-CT）进行扫描并进行组织病理学分析。微型CT显示了各组之间骨再生的不同方面。在对照组，UCS和OCS组中，外部愈伤组织体积占总骨体积的百分比分别为42.1％，13.0％和4.9％（p <0.05）。然而，具有间隙连通性的四肢的百分比分别为25％，12.5％和75％。在组织病理学评估中，OCS组显示组织良好且成熟的编织骨，在骨折部位具有周围软骨，而对照组则显示软骨形成，而在骨折部位没有骨成熟或骨化。同时，UCS组骨折部位仅充满纤维结缔组织，没有软骨内骨化和骨形成。提示MSC床单减少了外部愈伤组织的数量，OCS诱导了主要的骨愈合。

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